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Decrease in platelet reduced glutathione increases lipoxygenase activity and decreases vitamin E

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Lipids

Abstract

Unstimulated normal human blood platelets were treated with azodicarboxylic acidbis(dimethylamide) (diamide), a thiol-oxidizing agent. Oxygenated arachidonic acid (AA) metabolites, malondialdehyde (MDA), and tocopherols were then quantified by high-performance liquid chromatography (HPLC). Diamide treatment partially decreased the amount of reduced glutathione (GSH) content and induced a subsequent decrease in peroxidase activity. However, formation of 12-hydroxy-eicosatetraenoic acid (12-HETE), the end-product of lipoxygenation of AA, increased. Formation of MDA, a marker of overall lipid peroxidation, was also enhanced. Furthermore, platelet α-tocopherol, but not γ-tocopherol, significantly decreased. These results indicate that enhanced “basal” lipoxygenase activity, as a marker of specific AA oxygenation, may be linked to decreased platelet antioxidant status.

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Abbreviations

AA:

arachidonic acid

BHT:

butylated hydroxytoluene

GSH:

reduced glutathione

GSH-Px:

glutathione-peroxidase

(12 or 15)-HETE:

(12 or 15)-hydroxy-eicosatetraenoic acid

12-HHT:

12-hydroxy-heptadecatrienoic acid

(12 or 15)-HPETE:

(12 or 15)-hydroperoxy-eicosatetraenoic acid

MDA:

malondialdehyde

NADPH:

reduced nicotinamide adenine dinucleotide phosphate

OPT:

ortho-phthaldialdehyde

rp-HPLC:

reverse phase high-performance liquid chromatography

TBA:

thiobarbituric acid

TLC:

thin-layer chromatography

UV:

ultraviolet

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Authors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale U.205, Chimie Biologique INSA, 69621, Villeurbanne, France

    Catherine Calzada, Evelyne Véricel & Michel Lagarde

Authors
  1. Catherine Calzada
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  2. Evelyne Véricel
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  3. Michel Lagarde
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Calzada, C., Véricel, E. & Lagarde, M. Decrease in platelet reduced glutathione increases lipoxygenase activity and decreases vitamin E. Lipids 26, 696–699 (1991). https://doi.org/10.1007/BF02535616

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  • DOI: https://doi.org/10.1007/BF02535616

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