Abstract
Human platelet lipoxygenase activity toward several eicosaenoic acids was measured in intact cells as well as in subcellular fractions (cytosol and membranes). In whole platelets, the lipoxygenation of eicosaenoic acids was enhanced greatly by high concentrations of aspirin, which partially inhibit the peroxidase activity associated with the pathway. The lipoxygenation also was increased by arachidonic acid (AA) or its lipoxygenase product, 12-hydroxyperoxy-eicosatetraenoic acid (12-HPETE). Similarly, prostanoid precursors, dihomogammalinolenic (DHLA) and eicosapentaenoic (EPA) acids also were better converted by cyclooxygenase in the presence of AA or 12-HPETE. Among the eicosaenoic acids tested, EPA oxygenation was affected most.
Using cytosol or membranes as the lipoxygenase source instead of whole cells led to completely different results. AA exerted a competitive inhibition upon the other eicosaenoic acid oxygenation except that of EPA, for which a dual effect of AA was observed. This makes questionable the use of platelet subfractions for investigating lipoxygenase activity.
We conclude that platelet lipoxygenation of eicosaenoic acids appears peroxide-dependent, especially for apparent poor substrates like EPA. This might be relevant in respect to 12-HPETE, which is the main hydroperoxy derivative to be produced during platelet activation.
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References
Smith, J.B. (1980) Am. J. Pathol. 99, 742–804.
Bryant, R.W., and Bailey, J.M. (1980) Biochem. Biophys. Res. Commun. 92, 268–276.
Hamberg, M., Svensson, J., and Samuelsson, B. (1974) Proc. Natl. Acad. Sci. USA 71, 3824–3829.
Wallach, D.P., and Brown, V.R. (1981) Biochim. Biophys. Acta 663, 361–371.
Aharony, D., Smith, J.B., and Silver, M.J. (1984) in The Leukotrienes (Cahkvin, L.W., and Bailey, J.M., eds.) pp. 103–123, Academic Press, New York.
Nugteren, D.H. (1975) Biochim. Biophys. Acta 380, 299–307.
Ho, P.P.K., Walters, P., and Sullivan, H.R. (1977) Biochem. Biophys. Res. Commun. 76, 398–405.
Chang, W.C., Nakao, J., Orimo, H., and Murota, S. (1982) Biochem. J. 202, 771–776.
Lagarde, M., Croset, M., Authi, K.S., and Crawford, N. (1984) Biochem. J. 222, 495–500.
Aharony, D., Smith, J.B., and Silver, M.J. (1982) Biochim. Biophys. Acta 718, 193–200.
Croset, M., and Lagarde, M. (1983) Biochem. Biophys. Res. Commun. 112, 878–883.
Willis, A.L. (1981) Nutr. Rev. 39, 289–301.
Boukhchache, D., and Lagarde, M. (1982) Biochim. Biophys. Acta 713, 286–392.
Lagarde, M., Bryon, P.A., Guichardant, M., and Dechavanne, M. (1980) Thrombos. Res. 17, 581–588.
Menashi, S., Weintroub, M., and Crawford, N. (1981) J. Biol. Chem. 256, 4095–4101.
Wilhelm, T.E., Sankarappa, S.K., Van Rollins, M., and Sprecher, H. (1981) Prostaglandins 21, 323–332.
Siegel, M.I., McConnel, R.T., and Cuatrecasas, P. (1979) Proc. Natl. Acad. Sci. USA 76, 3774–3778.
Vericel, E., and Lagarde, M. (1980) Lipids 15, 472–474.
Katzenellenbogen, W., and Dobryszycka, W.A. (1959) Clin. Chim. Acta 4, 515–522.
Morita, I., Takahashi, R., Saito, Y., and Murota, S. (1983) J. Biol. Chem. 258, 10197–10199.
Culp, B.R., Titus, B.G., and Lands, W.E.M. (1979) Prostaglandin Med. 3, 269–278.
Vanderhoek, J.Y., Bryant, R.W., and Bailey, J.M. (1980) J. Biol. Chem. 255, 5596–5998.
Siegel, M.I., McConnel, R.T., Abrahams, S.L., Porter, N.A., and Cuatrecasas, P. (1979) Biochem. Biophys. Res. Commun. 89, 1273–1280.
Hamberg, M., and Hamberg, G. (1980) Biochem. Biophys. Res. Commun. 95, 1090–1097.
Neufeld, E.J., Wilson, D.B., Sprecher, H., and Majerus, P.W. (1983) J. Clin. Invest. 72, 214–220.
Boukhchache, D., and Lagarde, M. (1982) Biochem. Soc. Trans. 10, 237–238.
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Croset, M., Lagarde, M. Enhancement of eicosaenoic acid lipoxygenation in human platelets by 12-hydroperoxy derivative of arachidonic acid. Lipids 20, 743–750 (1985). https://doi.org/10.1007/BF02534397
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DOI: https://doi.org/10.1007/BF02534397