Skip to main content
SpringerLink
Log in

Alteration of liver microsomal proteins from rainbow trout (Salmo gairdneri) fed cyclopropenoid fatty acids

  • Communications
  • Published:
Lipids

Abstract

Rainbow trout were fed diets containing cyclopropenoid fatty acids (CPFA) at 50 and 300 ppm with appropriate controls fed CPFA-free diets. Treatment with CPFA altered the overall microsomal protein composition in a manner suggesting a reduction of high molecular weight components. One protein found in low concentration in controls appeared dominant in experimental animals, with the effect more pronounced as dietary levels of CPFA increased. The estimated molecular weight of this component was 41,500 daltons. Membrane fractions from CPFA-fed fish separated on a Bio-Gel P-150 column revealed a significant number of small molecular weight components that suggest degradation of microsomal proteins. These data suggest an alteration by CPFA of membrane protein composition.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Phelps, R.A., F.S. Shenstone, A.R. Kemmener and R.J. Evans, Poult. Sci. 44:358 (1965).

    PubMed  CAS  Google Scholar 

  2. Lee, D.J., J.H. Wales, J.L. Ayres and R.O. Sinnhuber, Cancer Res. 28:2313 (1968).

    Google Scholar 

  3. Malevski, Y., J.H. Wales, J.L. Ayres and M.W. Montgomery, J. Fish. Res. Board Can. 31:1397 (1974).

    CAS  Google Scholar 

  4. Struthers, B.J., J.H. Wales and R.O. Sinnhuber, Exp. Mol. Pathol. 23:164 (1975).

    Article  PubMed  CAS  Google Scholar 

  5. Roehm, J.N., D.J. Lee, J.H. Wales, S.D. Polityka and R.O. Sinnhuber, Lipids 5:80 (1970).

    Article  PubMed  CAS  Google Scholar 

  6. Scarpelli, D.G., D.J. Lee, R.O. Sinnhuber and M. Chiga, Cancer Res. 34:2984 (1974).

    PubMed  CAS  Google Scholar 

  7. Loveland, P.M., J.E. Nixon, N.E. Pawlowski, T.A. Eisele, L.M. Libbey and R.O. Sinnhuber, J. Environ. Pathol. Toxicol. 2:707 (1979).

    PubMed  CAS  Google Scholar 

  8. Schneider, E.L., S.P. Loke and D.T. Hopkins, J. Am. Oil. Chem. Soc. 45:585 (1968).

    CAS  Google Scholar 

  9. Laemmli, U.K., Nature 227:680 (1970).

    Article  PubMed  CAS  Google Scholar 

  10. Bradford, M., Anal. Biochem. 72:248 (1976).

    Article  PubMed  CAS  Google Scholar 

  11. Snedecor, G.W., and W.G. Cochran, in “Statistical Methods,” 6th Edition, Iowa State Univ. Press, Ames, IA, 1978, pp. 258–298.

    Google Scholar 

  12. Malevski, Y., M.W. Montgomery and R.O. Sinnhuber, J. Fish. Res. Board Can. 31:1093 (1974).

    CAS  Google Scholar 

  13. Taylor, S.K., M.W. Montgomery and D.J. Lee, J. Lipid Res. 14:643 (1973).

    PubMed  CAS  Google Scholar 

  14. Eisele, T.A., J.E. Nixon, N.E. Pawlowski and R.O. Sinnhuber, J. Environ. Pathol. Toxicol. 1:773 (1978).

    PubMed  CAS  Google Scholar 

  15. Van der Hoven, T.A., D.A. Haugen and M.J. Coon, Biochem. Biophys. Res. Commun. 60:569 (1974).

    Article  Google Scholar 

  16. Van der Hoven, T.A., and M.J. Coon, J. Biol. Chem. 249:6302, (1974).

    Google Scholar 

  17. Haugen, D.A., T.A. Van der Hoven and M.J. Coon, J. Biol. Chem. 250:3567 (1975).

    PubMed  CAS  Google Scholar 

  18. Haugen, D.A., and M.J. Coon, J. Biol. Chem. 251:7929 (1976).

    PubMed  CAS  Google Scholar 

  19. Yasukochi, Y., and B.B.S. Masters, J. Biol. Chem. 241:5337 (1976).

    Google Scholar 

  20. Coon, J.J., J.L. Vermillion, K.P. Vatsis, J.S. French, W.L. Dean and D.A. Haugen, in “Drug Metabolism Concepts,” edited by M. Jerina, ACS Symposium Series, No. 44, 1977, p. 46.

  21. Knapp, J.A., J.D. Dignom and H.W. Strobel, J. Biol. Chem. 252:437 (1977).

    PubMed  CAS  Google Scholar 

  22. Vermillion, J.L., and M.J. Coon, J. Biol. Chem. 253:2694 (1978).

    Google Scholar 

  23. Elcombe, C.R., and J.J. Lech, Toxicol. Appl. Pharmacol. 49:437 (1979).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Science, Oregon State University, 97331, Corvallis, OR

    Daniel P. Selivonchick & Janet L. Williams

  2. Department of Biochemistry and Biophysics, Oregon State University, 97331, Corvallis, OR

    Henry W. Schaup

Authors
  1. Daniel P. Selivonchick
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Janet L. Williams
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Henry W. Schaup
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Selivonchick, D.P., Williams, J.L. & Schaup, H.W. Alteration of liver microsomal proteins from rainbow trout (Salmo gairdneri) fed cyclopropenoid fatty acids. Lipids 16, 211–214 (1981). https://doi.org/10.1007/BF02535442

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02535442

Keywords

Search

Navigation