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Effect of detergents on in vitro 7α-hydroxycholesterol formation by rat liver microsomes

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Lipids

Abstract

Formation of 7α-hydroxycholesterol by rat liver microsomes was quantitated using a gas chromatograph-mass spectrometer (GC/MS) operated in selected ion monitoring (SIM) mode. Microsomes from normal rat livers incubated for different periods were found to yield increased 7α-hydroxycholesterol with time. This was also true when incubations contained Tween-80, but in this instance, the rate of 7α-hydroxycholesterol production was lower and dependent on the concentration of Tween used. Similarly, Triton X-100, Renex-30, Kyro EOB, Cutscum, and Emulgen 911 all lowered the formation of 7α-hydroxycholesterol by rat liver microsomes, whereas Triton WR-1339 stimulated its production. Analysis of data obtained from following the enzyme reaction over an extended period using an integrated Michaelis-Menten equation indicated the enzyme possesses a very significant affinity for the product (Ks>Kp). Similar analysis shows that Tween-80 is a noncompetitive inhibitor of the enzyme.

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Authors and Affiliations

  1. Department of Pathology, University of Pittsburgh School of Medicine, 15261, Pittsburgh, PA

    Ajit Sanghvi, Enrico Grassi, Candace Bartman & Warren F. Diven

Authors
  1. Ajit Sanghvi
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  2. Enrico Grassi
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  3. Candace Bartman
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  4. Warren F. Diven
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Sanghvi, A., Grassi, E., Bartman, C. et al. Effect of detergents on in vitro 7α-hydroxycholesterol formation by rat liver microsomes. Lipids 17, 644–649 (1982). https://doi.org/10.1007/BF02535372

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  • DOI: https://doi.org/10.1007/BF02535372

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