Skip to main content
SpringerLink
Log in

Cholesteryl ester hydrolase activity in adrenal homogenates from normal and essential fatty acid-deficient female rats

  • Published:
Lipids

Abstract

Cholesteryl ester hydrolase was assayed in adrenal homogenates from mature female rats fed a control (corn oil-containing) or essential fatty acid (EFA)-deficient diet. Cholesteryl ester of 16∶0, 18∶0, 18∶1, 18∶2(n−6), 20∶4(n−6) and 22∶4(n−6) were used as substrates. In control rats, the unsaturated esters were hydrolyzed more rapidly than the saturated esters and cholesteryl arachidonate was the preferred substrate of the six investigated; cholesteryl oleate elicited the highest activity in the deficient group. Polyunsaturated esters were hydrolyzed at a significantly lower rate by homogenates from EFA-deficient rats than by those from control animals. The esters of 18∶1, 18∶2(n−6) and 20∶4(n−6) were hydrolyzed more extenstively in relation to their concentrations in adrenal tissue than were cholesteryl esters of 16∶0, 18∶0 and 22∶4(n−6). This difference was more pronounced in control than in EFA-deficient rats. No simple relationship of adrenal cholesteryl ester hydrolase activity to ester fatty acid structure or to nutritional essentiality was evident.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EFA:

essential fatty acid(s)

x∶y(n−z):

fatty acid with x carbon atoms and y olefinic bonds with the terminal lefinic being z carbon atoms from the methyl group

ACTH:

adrenocorticotrophic hormone

References

  1. Sayers, C.T., Sayers, M.S., Fry, E.G., White, S., and Long, C.N.H. (1944) Yale J. Biol. Med. 16, 361–390.

    CAS  Google Scholar 

  2. Duncommun B., and Mach, R.S. (1949) Acta Endocrinol. 3, 17–26.

    Google Scholar 

  3. Glick, D., and Ochs, M.J. (1955) Endocrinology 56, 285–298.

    Article  PubMed  CAS  Google Scholar 

  4. Davis, W.W., and Garren, L.P. (1966) Biochem. Biophys. Res. Commun. 24, 805–810.

    Article  CAS  Google Scholar 

  5. Muraoka, R. (1965) Arch. Jpn. Chir. 34, 35–41.

    CAS  Google Scholar 

  6. Gidez, L.I., and Feller, E. (1969) J. Lipid Res. 10, 656–659.

    PubMed  CAS  Google Scholar 

  7. Walker, B.L., and Carney, J.A. (1971) Lipids 6, 797–804.

    Article  PubMed  CAS  Google Scholar 

  8. Young, A.K., and Walker, B.L. (1977) Lipids 13, 181–186.

    Article  Google Scholar 

  9. Hayashida, T., and Portman, O.W. (1959) Am. J. Physiol. 197, 893–896.

    CAS  Google Scholar 

  10. Hikasa, Y., Nagase, M., Matsuda, S., Satomura, K., Muraoka, R., and Fukada, H. (1966) Arch. Jpn. Chir. 35, 381–394.

    CAS  Google Scholar 

  11. Lamptey, M.S., and Walker, B.L. (1976) J. Nutr. 106, 86–93.

    PubMed  CAS  Google Scholar 

  12. Deykin, D., and Goodman D.S. (1962) J. Biol. Chem. 237, 3649–3656.

    PubMed  CAS  Google Scholar 

  13. Lowry, D.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  14. Eto, Y., and Suzuki, K. (1971) Biochim. Biophys. Acta 239, 293–311.

    PubMed  CAS  Google Scholar 

  15. Steel, R.G.D., and Torrie, J.H. (1960) in Principles and Procedures of Statistics, pp. 43–67, 99–132, McGraw-Hill Book Co., Inc., New York, NY.

    Google Scholar 

  16. Sgoutas, D.S. (1968) Biochim. Biophys. Acta 164, 317–326.

    PubMed  CAS  Google Scholar 

  17. Goller, H.J., and Sgoutas, D.S. (1970) Biochemistry 9, 4801.

    Article  PubMed  CAS  Google Scholar 

  18. Goller, H.J., Sgoutas, D.S., Ismail, I.A., and Gunstone, F.D. (1970) Biochemistry 9, 3072–3076.

    Article  PubMed  CAS  Google Scholar 

  19. Sgoutas, D.S. (1971) Biochim. Biophys. Acta 239, 469–474.

    PubMed  CAS  Google Scholar 

  20. Beckett, G.J., and Boyd, G.S. (1975) Eur. J. Biochem. 53, 335–342.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Nutrition, College of Biological Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Angela K. Young & Brian L. Walker

Authors
  1. Angela K. Young
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brian L. Walker
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Young, A.K., Walker, B.L. Cholesteryl ester hydrolase activity in adrenal homogenates from normal and essential fatty acid-deficient female rats. Lipids 17, 634–638 (1982). https://doi.org/10.1007/BF02535370

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02535370

Keywords

Search

Navigation