Abstract
The metabolism of labeled glucose, pyruvate and acetate was compared in adipocytes isolated from old, obese rats (>500 g) and young, lean rats (130–150 g). The larger cells from old, obese rats had markedly reduced rates of glucose, pyruvate and acetate conversion to glyceride-fatty acids, indicating that large cell fatty acid formation is reduced at some point beyond the entry of pyruvate and acetate into glucose metabolism. No evidence of a primary block in the pentose phosphate cycle of cells from old, obese rats was found. In spite of diminished glucose metabolism to several products in the large cells, both basal and insulin-stimulated rates of glyceride-glycerol synthesis from glucose and pyruvate were similar in each cell type. This indicates a relative diversion of carbon flow to α-glycerophosphate and reesterification in the large cells. Addition of low concentrations of glucose increased glyceride-fatty acid synthesis from acetate (both cell types) or pyruvate carbon (small cells), but decreased glyceride-glycerol synthesis from pyruvate carbon (both cell types). The acceleration of small cell fatty acid synthesis from pyruvate carbon by glucose and insulin was shown to be related to provision of NADPH from glucose metabolism in the pentose cycle. These studies indicate that, although the block in lipogenesis in adipocytes from old, obese rats appears to reside in the pathway of fatty acid synthesis itself, provision of additional α-glycerophosphate or NADPH from glucose metabolism may, under certain conditions, increase lipogenesis in cells from old, obese and young, lean rats.
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Abbreviations
- TMPD:
-
N,N,N′,N′-tetramethyl-p-phenylenediamine
- GSH:
-
reduced glutathione
- GSSG:
-
oxidized gluthathione
- KRB:
-
Krebs-Ringer bicarbonate
- ANOVA:
-
analysis of variance
References
DiGirolamo, M., and Rudman, D. (1968) Endocrinology 82, 1133–1141.
DiGirolamo, M., Howe, M.D., Esposito, J., Thurman, L., and Owens, J.L. (1974) J. Lipid Res. 15, 332–338.
Czech, M.P. (1976) J. Clin. Invest. 57, 842–851.
Richardson, D.K., and Czech, M.P. (1978) Am. J. Physiol. (Endocrinol. Metab. Gastrointest. Physiol. 3) 234, E182-E189.
Francendese, A.A., and DiGirolamo, M. (1981) Biochem. J. 194, 377–384.
Richardson, D.K., and Czech, M.P. (1979) Horm. Metab. Res. 11, 427–431.
Gellhorn, A., Benjamin, W., and Wagner, M. (1962) J. Lipid Res. 3, 314–319.
Rodbell, M. (1964) J. Biol. Chem. 239, 375–380.
Gliemann, J. (1965) Diabetes 14, 643–649.
May, J.M., Williams, R.H., and deHaën, C. (1978) J. Biol. Chem. 253, 686–690.
Bligh, E.G., and Dyer, W.J. (1959) Can. J. Biochem. Physiol. 37, 911–917.
Katz, J., and Rognstad, R. (1966) J. Biol. Chem. 241, 3600–3610.
Halperin, M.L., and Robinson, B.H. (1970) Biochem. J. 116, 235–240.
Kosower, N.S., Kosower, E.M., Wertheim, B., and Correa, W.S. (1969) Biochem. Biophys. Res. Commun. 37, 593–596.
Harris, J.W., and Biaglow, J.E. (1972) Biochem. Biophys. Res. Commun. 46, 1743–1749, 1972.
Eggleston, L.V., and Krebs, H.A. (1974) Biochem. J. 138, 425–435.
Lange, K., and Proft, E.R. (1970) Naunyn-Schmiedebergs Arch. Pharmacol. 267, 177–180.
Kather, H., Rivera, M., and Brand, K. (1972) Biochem. J. 128, 1097–1102.
Jamdar, S.C., and Osborne, L.J. (1981) Lipids 16, 829–834.
Livingston, J.N., and Lockwood, D.H. (1974) Biochem. Biophys. Res. Commun. 61, 989–996.
Olefsky, J.M. (1976) J. Clin. Invest. 57, 842–851.
Foley, J.E., Laursen, A.L., Sonne, O., and Gliemann, J. (1980) Diabetologia 19, 234–241.
Lewis, D.S., Masoro, E.J., and Yu, B.P. (1981) J. Lipid Res. 22, 1094–1101.
Robinson, B.H., and Halperin, M.L. (1970) Biochem. J. 116, 229–233.
Ballard, F.J., Hanson, R.W., and Leveille, G.A. (1967) J. Biol. Chem. 242, 2746–2750.
Reshef, L., Hanson, R.W. and Ballard, F.J. (1969) J. Biol. Chem. 244, 1994–2001.
Holm, G., Jacobsson, G., Bjorntorp, P., and Smith, U. (1976) J. Lipid Res. 16, 461–464.
Hartman, A.D., Cohen, A.I., Richane, C.J., and Hsu, T. (1971) J. Lipid Res. 12, 498–505.
Jamdar, S.C., Osborne, L.J., and Zeigler, J.A. (1981) Biochem. J. 194, 293–298.
Jamdar, S.C. (1978) Biochem. J. 170, 153–160.
Zinder, O., Arad, R., and Shapiro, B. (1967) Israel J. Med. Sci. 3, 787–791.
Halperin, M.L. (1970) Can. J. Biochem. 48, 1228–1233.
Katz, J., and Wals, P.S. (1971) Arch. Biochem. Biophys. 147, 405–418, 1971.
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May, J.M. Rat adipocyte utilization of different substrates: Effects of cell size and the control of lipogenesis. Lipids 17, 626–633 (1982). https://doi.org/10.1007/BF02535369
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DOI: https://doi.org/10.1007/BF02535369