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Effects of fish oil, corn oil and lard diets on lipid peroxidation status and glutathione peroxidase activities in rat heart

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Lipids

Abstract

In this study, we investigated the effect of various types of fats on heart lipid peroxidation status and on blood lipid parameters. Rats were fed either a low-fat diet (2.2% lard plus 2.2% corn oil), a corn oil diet (17%), a salmon oil diet (12.5%) supplemented with 4.5% corn oil, or a lard diet (15%) supplemented with 2% corn oil. All diets were supplemented with 1% cholesterol. Rats were fed for eight weeks. When compared with the low-fat diet, the salmon oil-diet intake resulted in a lower blood cholesterol, triglyceride and phospholipid concentrations (−50, −56 and −30%, respectively). Corn oil only tended to lower blood lipids; this decrease was significant for triglycerides only (−40%). The hypocholesterolemic effect of salmon oil diet is even more pronounced, if blood cholesterol values are compared with those of rats fed the lard diet. Heart lipid composition was not affected by dietary manipulations. Fatty acid composition of cardiac phosphatidylcholines and phosphatidylethanolamines, however, were altered by high-fat diets. In phosphatidylcholine, salmon oil induced a twelvefold decrease in the n−6/n−3 ratio and a 26% increase in the unsaturation index. For phosphatidylethanolamine, the n−6/n−3 ratio decreased 7.7-fold and the unsaturation index increased by 13%. A 50% decrease of the n−6/n−3 ratio was observed in animals fed the lard diet. Ultramicroscopic examination of ventricles revealed that those of the salmon oil group significantly accumulated lipofuscin-like or ceroid material, whereas this accumulation was barely detectable in hearts of the other groups. Seleniumdependent glutathione peroxidase activity tended to be the highest in hearts of rats fed the salmon oil diet; this increase is significant (+36% and +54% for total and specific activities, respectively), if values are compared with those of the rats fed the lard diet. Liver glutathione peroxidase and heart glutathione S-transferases activities remained unchanged. These results indicate that fish oil did not lower the selenium involved in glutathione peroxidase activity. This rules out that a deficiency in this enzyme was at the origin of heart lipofuscinosis. Also, it is concluded that the n−6/n−3 ratio of the diet is likely more determinant in the alteration of heart lipid peroxidation status than is the polyunsaturated/saturated ratio.

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Abbreviations

ALAT:

alanine aminotransferase

ASAT:

aspartate aminotransferase

FAME:

fatty acid methyl ester

GSH-PX:

selenium-dependent glutathione peroxidase

GST:

glutathione S-transferase

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PL:

phospholipid

PUFA:

polyunsaturated fatty acid

TG:

triglyceride

ANOVA:

analysis of variance

GLC:

gas liquid chromatography

HPLC:

high performance liquid chromatography

P/S:

polyunsaturated/saturated

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Authors and Affiliations

  1. INSERM U 130, 18 Avenue Mozart, 13009, Marseille, France

    Gilles Nalbone, Jeannie Leonardi, Elise Termine, Paulette Lechene & Huguette Lafonf

  2. Laboratoire Central, (Prs. J. Pastor and A.M. Pauli), Hopital Ste. Marguerite, 13009, Marseille, France

    Henri Portugal & Anne-Marie Pauli

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  1. Gilles Nalbone
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  2. Jeannie Leonardi
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  3. Elise Termine
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  4. Henri Portugal
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  5. Paulette Lechene
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  6. Anne-Marie Pauli
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  7. Huguette Lafonf
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Nalbone, G., Leonardi, J., Termine, E. et al. Effects of fish oil, corn oil and lard diets on lipid peroxidation status and glutathione peroxidase activities in rat heart. Lipids 24, 179–186 (1989). https://doi.org/10.1007/BF02535232

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  • DOI: https://doi.org/10.1007/BF02535232

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