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Incorporation of arachidonic, dihomogamma linolenic and eicosapentaenoic acids into cultured V79 cells

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Lipids

Abstract

The uptake and distribution of three common dietary polyunsaturated fatty acids was studied using Chinese hamster lung fibroblasts (V79 cells). Treatment of V79 cells with arachidonic (20∶4), eicosapentaenoic (20∶5) and dihomogammalinolenic (20.3) acids for 24 hr produced a marked uptake of 20∶3 and 20∶4, both of which were assimilated to a considerably greater degree than 20∶5. All polyunsaturated fatty acids were incorporated primarily into phospholipids; however, there were considerable differences in their distribution into individual phospholipid species. Although 20∶4 was incorporated primarily into phosphatidylcholine, 20∶3 entered largely into phosphatidylethanolamine and phosphatidylglycerol, and 20∶5 was distributed about equally between phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol. A marked conversion of 20∶3 to 20∶4 was found after 24 hr and, in several phospholipids, there was as much derived-radiolabeled 20∶4 as there was radiolabeled 20∶3. There was little evidence of 20∶4 and 20∶5 metabolism. V79 cells undergo substantial changes in phospholipid fatty acid composition following supplementation with these polyunsaturated fatty acids; however, these fatty acids are assimilated to different degrees and their distribution among cellular phospholipids is distinct, suggesting incorporation via independent mechanisms.

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Abbreviations

CL:

cardiolipin

FAME:

fatty acid methyl ester

LPC:

lysophosphatidylcholine

PA:

phosphatidic acid

PBS:

phosphate buffered saline

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PG:

pnosphatidylglycerol

PI:

phosphatidylinositol

PS:

phosphatidylserine

PUFA:

polyunsaturated fatty acids

SM:

sphingomyelin

GC:

gas chromatography

HPLC:

high performance liquid chromatography

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Institute for Toxicology and Department of Pathology, University of Southern California, School of Pharmacy, 1985 Zonal Avenue, 90033, Los Angeles, CA

    Klaus U. Weithmann, Hazel Peterson & Alex Sevanian

Authors
  1. Klaus U. Weithmann
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  2. Hazel Peterson
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  3. Alex Sevanian
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Weithmann, K.U., Peterson, H. & Sevanian, A. Incorporation of arachidonic, dihomogamma linolenic and eicosapentaenoic acids into cultured V79 cells. Lipids 24, 173–178 (1989). https://doi.org/10.1007/BF02535231

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  • DOI: https://doi.org/10.1007/BF02535231

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