Abstract
A convenient and efficient procedure has been devised for the large-scale preparation of asialo-GM1 from bovine brain gangliosides. The procedure relies on the complete desialylation of brain ganglio-sides, consisting primarily of GM1, GD1a, GD1b and GT1b, by mild formic acid hydrolysis (0.1 N, 100 C; 2 hr). Following the hydrolysis step, asialo-GM1 can be isolated and purified by Folch partitioning and Iatrobeads column chromatography, with an overall yield of more than 50%.
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The ganglioside nomenclature used is that of Svennerholm (1).
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Kasai, N., Sillerud, L.O. & Yu, R.K. A convenient method for the preparation of asialo-GM1 . Lipids 17, 107–110 (1982). https://doi.org/10.1007/BF02535184
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DOI: https://doi.org/10.1007/BF02535184