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Lipids and fatty acids of the horseshoe crabsTachypleus gigas andCarcinoscorpius rotundicauda

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Lipids

Abstract

Total lipids from hepatopancreas of the horseshoe crabs,Tachypleus gigas andCarcinoscorpius rotundicauda, obtained in 7.6 and 3.3% wet weight respective yields, were fractionated by various chromatographic techniques and identified by gas-liquid chromatography and spectroscopic methods. Fatty acid-containing lipids were rich in 16∶0 (8.0–25%), 18∶1ω9 (6.9–22%) and 18∶2ω6 (6.8–18.5%); appreciable amounts of 16∶1ω7, 18∶3ω3, 20∶5ω3 and 22∶6ω3 were also present. The level of 26∶0 in the hydrocarbon fractions was unusually high (64 and 68%). Carbon chain lengths of major wax esters were 44, 46 and 48 forT. gigas and 38, 40 and 42 forC. rotundicauda. 1-O-Alkyl diglycerides were 7.2 and 9.1% of the total lipids in the two species and contained 14∶0(20%), 16∶0(60%) and 18∶0(20%) alkyl chains along with a relatively higher percentage (32–35%) of saturated fatty acids. High levels of cholesterol (>50% of total sterol) in the free and combined state were encountered in both samples, phospholipid contents being 40 and 35%, respectively, and contained highest levels of unsaturated fatty acids.

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Abbreviations

ECL:

equivalent chain length

GLS:

gas-liquid chromatography

NMID:

nonmethylene interrupted dienoic

PL:

phospholipids

RRT:

relative retention times

TAG:

triacylglycerol

TLC:

thin-layer chromatography

TMS:

tetramethylsilane

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Authors and Affiliations

  1. Indian Institute of Chemical Biology, 700 032, Calcutta, India

    Satyesh C. Pakrashi, Pradeep K. Duffa & Basudeb Achari

  2. Department of Marine Science, Calcutta University, 700 019, Calcutta, India

    Suniti Misra & Amalesh Choudhury

  3. Department of Chemistry, Bose Institute, 700 009, Calcutta, India

    Subhashis Chattopadhyay & Amitabha Ghosh

Authors
  1. Satyesh C. Pakrashi
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  2. Pradeep K. Duffa
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  3. Basudeb Achari
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  4. Suniti Misra
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  5. Amalesh Choudhury
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  6. Subhashis Chattopadhyay
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  7. Amitabha Ghosh
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Pakrashi, S.C., Duffa, P.K., Achari, B. et al. Lipids and fatty acids of the horseshoe crabsTachypleus gigas andCarcinoscorpius rotundicauda . Lipids 24, 443–447 (1989). https://doi.org/10.1007/BF02535154

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  • DOI: https://doi.org/10.1007/BF02535154

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