Skip to main content
SpringerLink
Log in

Intestinal cholesterol uptake from phospholipid vesicles and from simple and mixed micelles

  • Published:
Lipids

Abstract

This study was undertaken in vitro to examine the rat jejunal uptake of cholesterol from phospholipid vesicles and from mixed bile salt micelles, under conditions of low effective resistance of the intestinal unstirred water layer. Cholesterol uptake Jd, occurred from vesicles only when the cholesterol: phospholipid ratio was high. The addition of phospholipid (PL) to micelles comprising 20 mM taurodeoxycholic acid (TDC) extended the concentration of cholesterol, beyond which the relationship between cholesterol concentration and uptake remained linear. When the concentration of cholesterol in the bulk phase was held constant and the concentration of TDC or of PL added to the TDC was increased, there was a decline in cholesterol uptake; this effect was masked when the concentration of TDC was high, or when higher concentrations of PL were added to the mixed micelle. When increasing concentrations of palmitic acid were added to mixed micelles composed of cholesterol, TDC and PL, the uptake of cholesterol decreased; in contrast, cholesterol uptake progressively increased when palmitic acid was added to simple TDC micelles. The results suggest that the mechanism responsible for cholesterol uptake may vary, depending on the nature of the constituents of the micelle, and it is proposed that PL inhibits the intestinal uptake of cholesterol by altering the partitioning of cholesterol out of the micelle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hoffman, N.E. (1970) Biochim. Biophys. Acta 196, 193–203.

    Article  PubMed  CAS  Google Scholar 

  2. Watt, S.M., and Simmonds, W.J. (1971) Biochim. Biophys. Acta 225, 347–355.

    Article  PubMed  CAS  Google Scholar 

  3. Feldman, E.B., and Cheng, C.Y. (1975) Am. J. Nutr. 28, 692–698.

    Google Scholar 

  4. O'Doherty, P.J.A., Kakis, G., and Kuksis, A. (1973) Lipids 8, 249–255.

    Article  PubMed  Google Scholar 

  5. Tso, P., Balint, J.A., and Simmonds, W.J. (1976) Gastroenterology 13, 1362–1367.

    Google Scholar 

  6. Tso, P., Lam, J., and Simmonds, W.J. (1978) Biochim. Biophys. Acta 538, 364–372.

    Google Scholar 

  7. Rampone, A.J. (1972) J. Physiol. 227, 889–898.

    PubMed  CAS  Google Scholar 

  8. Schiff, E.R., Small, N.C., and Dietschy, J.M. (1972) J. Clin. Invest. 51, 1351–1362.

    PubMed  CAS  Google Scholar 

  9. Saunders, D.R., and Dawson, A.M. (1963) Gut 4, 254–260.

    PubMed  CAS  Google Scholar 

  10. Saunders, D.R., and Sillery, J. (1976) Lipids 11, 830–832.

    Article  PubMed  CAS  Google Scholar 

  11. Rodgers, J.B., and O'Connor, P.J. (1975) Biochim. Biophys. Acta 409, 192–200.

    PubMed  CAS  Google Scholar 

  12. O'Connor, P.J., and Rodgers, J.B. (1976) Biochim. Biophys. Acta 450, 402–409.

    PubMed  Google Scholar 

  13. Rodgers, J.B., Fondacaro, J.D., and Kot, J. (1977) J. Lab. Clin. Med. 89, 147–152.

    PubMed  CAS  Google Scholar 

  14. O'Connor, P.J., Loiudice, T.A., Bochenek, W., and Rodgers, J.B. (1978) Am. J. Dig. Dis. 23, 316–320.

    Article  PubMed  Google Scholar 

  15. Fondacaro, J.D., and Rodgers, J.B. (1978) Dig. Dis. Sci. 23, 12–17.

    Article  CAS  Google Scholar 

  16. Rampone, A.J. (1973) J. Physiol. 229, 495–503.

    PubMed  CAS  Google Scholar 

  17. Rampone, A.J. (1973) J. Physiol. 229, 505–514.

    PubMed  CAS  Google Scholar 

  18. Carey, M.C., and Small, D.M. (1972) Arch. Int. Med. 130, 506–527.

    Article  CAS  Google Scholar 

  19. Mazen, N.A., Kwasnick, R.F., Carey, MC., and Benedek, G.B. (1977) in Micellization, Solubilization and Microemulsions (Mittal, K.L., ed.) Vol. 1, pp. 388–401, Plenum Press, New York, NY

    Google Scholar 

  20. Feldman, E.B. (1968) Biochem. Med. 2, 136–144.

    Article  CAS  Google Scholar 

  21. Lukie, B.E., Westergaard, H., and Dietschy, J.M. (1974) Gastroenterology 67, 652–661.

    PubMed  CAS  Google Scholar 

  22. Westergaard, H., and Dietschy, J.M. (1974) J. Clin. Invest. 54, 718–732.

    PubMed  CAS  Google Scholar 

  23. Sallee, V.L. (1974) J. Lipid Res. 15, 56–64.

    PubMed  CAS  Google Scholar 

  24. Helenius, A., and Simons, K. (1975) Biochim. Biophys. Acta 415, 29–79.

    PubMed  CAS  Google Scholar 

  25. Sallee, V.L. (1978) J. Lipid Res. 19, 207–214.

    PubMed  CAS  Google Scholar 

  26. Ammar, H.V., Thomas, P.J., and Phillips, S.F. (1978) Lipids 14, 395–400.

    Google Scholar 

  27. Rampone, A.J., and Lang, L.R. (1977) Biochim. Biophys. Acta 486, 500–510.

    PubMed  CAS  Google Scholar 

  28. Tyrrell, D.A., Ryman, B.E., Keetan, B.R., and Dubowitz, V. (1976) Br. Med. J. 2, 88.

    Article  PubMed  CAS  Google Scholar 

  29. Dapergolos, G., Neerunjun, E.D., and Gregoriadis, G. (1976) FEBS Lett. 63, 235–239.

    Article  Google Scholar 

  30. Patel, H.M., and Ryman, B.E. (1976) FEBS Lett. 62, 60–63.

    Article  PubMed  CAS  Google Scholar 

  31. Patel, H.M., and Ryman, B.E. (1977) Biochem. Soc. Trans. 5, 1054–1055.

    PubMed  CAS  Google Scholar 

  32. Patel, H.M., and Ryman, B.E. (1977) Biochem. Soc. Trans. 5, 1739–1741.

    PubMed  CAS  Google Scholar 

  33. Patel, H.M., Harding, N.G.L., Logue, F., Kesson, C., MacCuish, A.C., McKenzie, J.C., Ryman, B.E., and Scobie, I. (1978) Biochem. Soc. Trans. 6, 785–789.

    Google Scholar 

  34. Ryman, B.E., Jewkes, R.F., Jeyasingh, K., Osborne, M.P., Patel, H.M., Richardson, V.J., Tattersall, M.H.N., and Tyrrell, D.A. (1978) An. N.Y. Acad. Sci. 308, 281–307.

    CAS  Google Scholar 

  35. Gregoriadis, G. (1976) N. Engl. J. Med. 295, 704–710, 765–779.

    Article  PubMed  CAS  Google Scholar 

  36. Gregoriadis, G. (1976) in Methods in Enzymology (Mosbach, K., ed.) Vol. 44, pp. 698–709, Academic Press, New York, NY.

    Google Scholar 

  37. Westergaard, H., and Dietschy, J.M. (1976) J. Clin. Invest. 58, 97–108.

    Article  PubMed  CAS  Google Scholar 

  38. Thomson, A.B.R. (1980) J. Lipid Res. 2, 1097–1107.

    Google Scholar 

  39. Sallee, V.L., Wilson, F.A., and Dietschy, J.M. (1972) J. Lipid Res. 13, 184–192.

    PubMed  CAS  Google Scholar 

  40. Singh, A., Balint, J.A., Edmonds, R.H., and Rodgers, J.B. (1972) Biochim. Biophys. Acta 260, 708–715.

    PubMed  CAS  Google Scholar 

  41. Ponz de Leon, M., Carulli, N., Loria, P., Lori, R., and Zironi, F. (1979) Gastroenterology 77, 223–230.

    PubMed  CAS  Google Scholar 

  42. Small, D.M. (1971) in The Bile Acids, Chemistry, Physiology and Metabolism (Nair, D.P., and Kritchevsky, D., eds.) Vol. 1, pp. 249–356, Plenum Publishing Co., New York, NY.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine, University of Alberta, T6G 2G3, Edmonton, Canada

    A. B. R. Thomson & L. Cleland

Authors
  1. A. B. R. Thomson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Cleland
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Thomson, A.B.R., Cleland, L. Intestinal cholesterol uptake from phospholipid vesicles and from simple and mixed micelles. Lipids 16, 881–887 (1981). https://doi.org/10.1007/BF02534992

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02534992

Keywords

Search

Navigation