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Analysis of conjugated bile acids by high performance liquid chromatography and mass spectrometry

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Lipids

Abstract

Because of the known advantages of coupling high performance liquid chromatography with mass spectrometry (HPLC-MS) in biological fluids, studies on the reversed-phase HPLC-MS system for direct analysis of conjugated bile acids in human bile samples are described. Ten samples of gallbladder bile of apparently healthy subjects were examined. The amounts of each tauro- and glycoconjugated bile acid as trifluoracetate were determined by mass fragmentography. Quantitation of at least 1 ng of each bile acid was possible.

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Abbreviations

TUDCA:

tauroursodeoxycholic acid

TCA:

taurocholic acid

TCDCA:

taurochenodeoxycholic acid

TDCA:

taurodeoxycholic acid

TLCA:

taurolithocholic acid

GCA:

glycocholic acid

GCDCA:

glycochenodeoxycholic acid

GDCA:

glycodeoxycholic acid

GLCA:

glycolithocholic acid

TBA:

total bile acids

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Authors and Affiliations

  1. Istituto di Patologia Medica, Catholic University, Rome, Italy

    G. Mingrone & A. V. Greco

  2. Department of Chemistry, Istituto Superiore di Sanità, Rome, Italy

    L. Boniforti

  3. Istituto S. Gallicano, Rome, Italy

    S. Passi

Authors
  1. G. Mingrone
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  2. A. V. Greco
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  3. L. Boniforti
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  4. S. Passi
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Mingrone, G., Greco, A.V., Boniforti, L. et al. Analysis of conjugated bile acids by high performance liquid chromatography and mass spectrometry. Lipids 18, 90–95 (1983). https://doi.org/10.1007/BF02534697

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  • DOI: https://doi.org/10.1007/BF02534697

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