Abstract
Bile acids are important end products of cholesterol metabolism, having been shown to serve as signaling molecules and intermediates between the host and the gut microbiota. Here we describe a robust and accurate method using ultrahigh-pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the quantification of bile acids in stool/cecal and tissue samples.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Falany CN, Xie XW, Wheeler JB et al (2002) Molecular cloning and expression of rat liver bile acid CoA ligase. J Lipid Res 43(12):2062–2071
Falany CN, Johnson MR, Barnes S, Diasio RB (1994) Glycine and taurine conjugation of bile-acids by a single enzyme - molecular-cloning and expression of human liver bile-acid CoA-amino acid N-acyltransferase. J Biol Chem 269(30):19375–19379
Hofmann AF, Hagey LR (2008) Bile acids: chemistry, pathochemistry, biology, pathobiology, and therapeutics. Cell Mol Life Sci 65(16):2461–2483
Urdaneta V, Casadesus J (2017) Interactions between bacteria and bile salts in the gastrointestinal and hepatobiliary tracts. Front Med 4:163
Begley M, Hill C, Gahan CGM (2006) Bile salt hydrolase activity in probiotics. Appl Environ Microbiol 72(3):1729–1738
Begley M, Gahan CGM, Hill C (2005) The interaction between bacteria and bile. FEMS Microbiol Rev 29(4):625–651
Devlin AS, Fischbach MA (2015) A biosynthetic pathway for a prominent class of microbiota-derived bile acids. Nat Chem Biol 11(9):685
Li F, Jiang CT, Krausz KW et al (2013) Microbiome remodelling leads to inhibition of intestinal farnesoid X receptor signalling and decreased obesity. Nat Commun 4:2384
Jiang CT, Xie C, Lv Y et al (2015) Intestine-selective farnesoid X receptor inhibition improves obesity-related metabolic dysfunction. Nat Commun 6:1016
Ridlon JM, Kang DJ, Hylemon PB, Bajaj JS (2014) Bile acids and the gut microbiome. Curr Opin Gastroenterol 30(3):332–338
Li TG, Chiang JYL (2014) Bile acid signaling in metabolic disease and drug therapy. Pharmacol Rev 66(4):948–983
Chiang JYL (2013) Bile acid metabolism and signaling. Compr Physiol 3(3):1191–1212
Mertens KL, Kalsbeek A, Soeters MR, Eggink HM (2017) Bile acid signaling pathways from the enterohepatic circulation to the central nervous system. Front Neurosci 11:617
Dutta M, Cai J, Gui W, Patterson AD (2019) A review of analytical platforms for accurate bile acid measurement. Anal Bioanal Chem 411(19):4541–4549
Tian Y, Cai JW, Gui W et al (2019) Berberine directly affects the gut microbiota to promote intestinal farnesoid X receptor activation. Drug Metab Dispos 47(2):86–93
Perwaiz S, Tuchweber B, Mignault D, Gilat T, Yousef IM (2001) Determination of bile acids in biological fluids by liquid chromatography-electrospray tandem mass spectrometry. J Lipid Res 42(1):114–119
Kakiyama G, Muto A, Takei H et al (2014) A simple and accurate HPLC method for fecal bile acid profile in healthy and cirrhotic subjects: validation by GC-MS and LC-MS. J Lipid Res 55(5):978–990
Tian Y, Zhang LM, Wang YL, Tang HR (2012) Age-related topographical metabolic signatures for the rat gastrointestinal contents. J Proteome Res 11(2):1397–1411
Han J, Liu Y, Wang RX, Yang JC, Ling V, Borchers CH (2015) Metabolic profiling of bile acids in human and mouse blood by LC-MS/MS in combination with phospholipid-depletion solid-phase extraction. Anal Chem 87(2):1127–1136
Sarafian MH, Lewis MR, Pechlivanis A et al (2015) Bile acid profiling and quantification in biofluids using ultra-performance liquid chromatography tandem mass spectrometry. Anal Chem 87(19):9662–9670
Parasuraman S, Raveendran R, Kesavan R (2010) Blood sample collection in small laboratory animals. J Pharmacol Pharmacother 1(2):87–93
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Tian, Y., Cai, J., Allman, E.L., Smith, P.B., Patterson, A.D. (2021). Quantitative Analysis of Bile Acid with UHPLC-MS/MS. In: Markowitz, J. (eds) Translational Bioinformatics for Therapeutic Development. Methods in Molecular Biology, vol 2194. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0849-4_15
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0849-4_15
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0848-7
Online ISBN: 978-1-0716-0849-4
eBook Packages: Springer Protocols