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Quantitative Analysis of Bile Acid with UHPLC-MS/MS

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Translational Bioinformatics for Therapeutic Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2194))

Abstract

Bile acids are important end products of cholesterol metabolism, having been shown to serve as signaling molecules and intermediates between the host and the gut microbiota. Here we describe a robust and accurate method using ultrahigh-pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the quantification of bile acids in stool/cecal and tissue samples.

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Correspondence to Andrew D. Patterson .

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Tian, Y., Cai, J., Allman, E.L., Smith, P.B., Patterson, A.D. (2021). Quantitative Analysis of Bile Acid with UHPLC-MS/MS. In: Markowitz, J. (eds) Translational Bioinformatics for Therapeutic Development. Methods in Molecular Biology, vol 2194. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0849-4_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0849-4_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0848-7

  • Online ISBN: 978-1-0716-0849-4

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