Studies on the half-life and the distribution of stearoyl-CoA desaturase in the housefly

Abstract

The half-life of the stearoyl-CoA desaturase was determined by using houseflies injected with puromycin. The LD₅₀ for puromycin was determined to be 1.55 μ g/mg body wt for both sexes. By following the decay in specific activity of the desaturase after puromycin injection, the half-life of this enzyme in 4-day-old females was calculated to be 9.35 hr, while in 1-day-old females it was 3.38 hr. The inhibition curve for the 3-day-old males never reached 100%, and a biphasic curve was observed. The early phase resulted in a half-life of the desaturase of 2.41 hr, while the latter phase had a half-life of 8.45 hr. In general, it appeared that the half-life of the desaturase increased with age in either sex. Studies on the distribution of desaturase activity within the housefly showed that most of the activity was present in the integument; however, in the female, the onset of ovarian development seemed to shift the distribution of desaturase from the integument, towards the internal tissues. In the male, there appeared to be equal amounts of desaturase in integument and the fat body plus internal tissues at day 2, but by day 5 most of the activity was associated with the integument. The high specific activity and high percentage of total desaturase activity associated with integument coupled with the shift of distribution of desaturase in females during the onset of ovarian development may be indicative of a close correlation of the desaturase with alkene synthesis and possibly sex pheromone production.