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Growth of a sterol auxotroph derived fromSaccharomyces cerevisiae on chemically synthesized derivatives of cholesterol possessing side-chain modifications

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Lipids

Abstract

A number of cholesterol derivatives were analyzed for their ability to satisfy bulk membrane and high-specificity sparking requirements of a yeast sterol auxotroph (RD5-R) (Rodriguez, R. J., Taylor, F. R., and Parks, L. W. [1982], Biochem. Biophys. Res. Commun. 106, 435–441). Substitution of hydrogen by bromine or iodine at C-26 or substitution of C26-methyl by bromine enabled the resulting sterol to satisfy bulk or sparking functions. The presence of a side-chain hydroxyl or keto group at C-25 on a 26-norcholesterol completely abolished the ability of cholesterol to satisfy either sterol requirement. Growth studies revealed that, while the oxygenated cholesterol derivatives were not growth-supportive of RD5-R, they were not growth-inhibitory.

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Author notes

  1. L. W. Parks

    Present address: Department of Microbiology, Oregon State University, 97331-3804, Corvallis, OR

Authors and Affiliations

  1. Department of Microbiology, Oregon State University, 97331-3804, Corvallis, OR

    R. J. Rodriguez

  2. The Worcester Foundation for Experimental Biology, 222 Maple Avenue, 01545, Shrewsbury, MA

    T. A. Arunachalam & E. Caspi

Authors
  1. R. J. Rodriguez
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  2. T. A. Arunachalam
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  3. L. W. Parks
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  4. E. Caspi
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Rodriguez, R.J., Arunachalam, T.A., Parks, L.W. et al. Growth of a sterol auxotroph derived fromSaccharomyces cerevisiae on chemically synthesized derivatives of cholesterol possessing side-chain modifications. Lipids 18, 772–775 (1983). https://doi.org/10.1007/BF02534634

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  • DOI: https://doi.org/10.1007/BF02534634

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