Abstract
A method for the deacylation of galactosylceramide I3-sulphate using aqueous methanolic KOH is described. The combination of a relatively low concentration of the alkali (0.3 M) and a moderate reaction temperature (reflux point of 90% methanol) results in the formation of galactosylsphingosine I3-sulphate in consistently high yields (61%) with a minimum of side reactions. The product was purified by column chromatography and its identity established by thin layer chromatography, infrared spectroscopy, determination of galactose content and organic sulphate assay using established methods or their modifications.
Similar content being viewed by others
References
Radin, N.S. (1974) Lipids 9, 358–360.
Nonaka, G., Kishimoto, Y., Seyama, Y., and Yamakawa, T. (1979) J. Biochem. 85, 511–518.
Kushwaha, S.C., and Kates, M. (1981) Lipids 16, 372–373.
Kean, E.L. (1968) J. Lipid Res. 9, 319–327.
Stoffyn, P., and Stoffyn, A. (1963) Biochim. Biophys. Acta 70, 218–220.
Klenk, E., and Harle, R. (1928) Z. Physiol. Chem. 178, 221.
Author information
Authors and Affiliations
About this article
Cite this article
Koshy, K.M., Boggs, J.M. A high-yield saponification of galactosylceramide I3-sulfate. Lipids 17, 998–1000 (1982). https://doi.org/10.1007/BF02534598
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02534598