Abstract
The microsomal stearoyl-CoA desaturase system was examined in both the Morris hepatoma 7288CTC, maintained in the host Buffalo strain rat, and the Morris hepatoma 7288C, maintained in tissue culture. In vitro examination shows the stearoyl-CoA desaturase system to be similar in the 2 tissues. Both show extremely low overall stearoyl-CoA desaturase activity, having 4% and 8% of normal liver values respectively. Examination of the electron transport system showed both tissues have decreased electron transport components cytochrome b5 and cytochrome b5 reductase. Particularly noticeable were the extremely low levels of cytochrome b5 (2% compared with normal liver). Microsomes from both tissues showed a decreased ability to reduce an artificial electron acceptor, cytochrome c. With the low levels of cytochrome b5 observed in these tissues, the low levels of overall desaturase activity may be caused by lack of terminal enzyme, lack of sufficient cytochrome b5, or both. Analysis of the stearoyl-CoA desaturase system in cultured hepatoma cells suggests that these cells are similar to the host-grown tumor in this respect and may be used as a model in further examinations of the stearoyl-CoA desaturase system.
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References
Howard, B., Butler, J.D., and Bailey, J.M. (1972) in Tumor Lipids: Biochemistry and Metabolism (Wood, R., ed.) pp. 200–214, American Oil Chemists’ Society, Champaign IL.
Van Hoeven, R.P., and Emmelot, P. (1972) in Tumor Lipids: Biochemistry and Metabolism (Wood, R., ed.) pp. 126–138, American Oil Chemists’ Society, Champaign, IL.
Waite, M., Parce, B., Morton, R., Cunningham, C., and Morris, H.P. (1977) Cancer Res. 37, 2902–2908.
Reitz, R.C., Thompson, J.A., and Morris, H.P. (1977) Cancer Res. 37, 561–567.
Morton, R., Cunningham, C., Jester, R., Waite, M., Miller, N., and Morris, H.P. (1976) Cancer Res. 36, 3246–3254.
Morton, R.E., Hartz, J.W., Reitz, R.C., Waite, M., and Morris, H.P. (1979) Biochim. Biophys. Acta 573, 321–331.
Lee, T., Stephens, N., and Snyder, F. (1974) Cancer Res. 34, 3270–3273.
Prasad, M.R., and Joshi, V.C. (1979) Lipids 14, 413–415.
Chiappe, L., Mercuri, O., and de Thomas, M.E. (1974) Lipids 9, 489–490.
Raju, P.K. (1974) Lipids 9, 795–797.
Wood, R. (1973) Lipids 8, 690–701.
Wood, R., Falch, J., and Wiegand, R.D. (1975) Lipids 10, 202–207.
Wood, R. (1975) Lipids 10, 736–745.
Weigand, R., and Wood, R. (1975) Lipids 10, 194–201.
Jones, P.D., Holloway, P.W., Peluffo, R.O., and Wakil, S.J. (1969) J. Biol. Chem. 249, 744–754.
Omura, J., and Sato, R. (1964) J. Biol. Chem. 239, 2370–2378.
Oshino, N., and Sato, R. (1972) Arch. Biochem. Biophys. 149, 369–377.
Jones, P.D., and Wakil, S.J. (1967) J. Biol. Chem. 242, 5267–5273.
Cornall, A.G., Bardawill, G.J., and David, M.M. (1949) J. Biol. Chem. 177, 751–755.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.
DeWys, W. (1970) Cancer Res. 30, 2816–2818.
Joshi, V.C., and Aranda, L.P. (1979) J. Biol. Chem. 254, 11779–11782.
Shenkman, J.B., Jansson, I., and Robie-Suh, K.M. (1976) Life Sciences 19, 611–624.
Keyes, S.R., Alfano, J.A., Jansson, I., and Cinti, D.L. (1979) J. Biol. Chem. 254, 7778–7784.
Upreti, G., de Antueno, R.J., and Wood, R.D. (1983) J. Natl. Canc. Inst. 70, 567–573.
Watson, J.A. (1972) in Tumor Lipids: Biochemistry and Metabolism (Wood, R., ed.) p. 34–53, American Oil Chemists’ Society, Champaign, IL.
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Zoeller, R.A., Wood, R. Analysis of the stearoyl-CoA desaturase system in the Morris hepatoma 7288C and 7288CTC. Lipids 19, 488–491 (1984). https://doi.org/10.1007/BF02534480
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DOI: https://doi.org/10.1007/BF02534480