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A colorimetric microdetermination of peroxide values utilizing aluminum chloride as the catalyst

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Lipids

Abstract

A colorimetric microassay is described for the determination of lipid hydroperoxides. Hydroperoxides are reacted with potassium iodide in the presence of an acid catalyst and liberated iodine is measured. Aluminum chloride, an alcohol-soluble Lewis acid, is used as catalyst. Liberated iodine is measured colorimetrically at 560 nm after addition of starch in 0.01 N hydrochloric acid. The range of the measurement was 0.05–0.5 μmol of hydroperoxides.

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References

  1. Lea, C.H., Proc. Soc. London Ser. B, 108:175 (1931).

    Article  CAS  Google Scholar 

  2. Wheeler, D.H., Oil Soap (Chicago) 9:89 (1932).

    CAS  Google Scholar 

  3. The Standard Methods of Jpn. Oil Chem. Soc. 2.4.12–71.

  4. Lips, A., R.A. Chapman and W.D. McFarlane, Oil Soap (Chicago) 20:240 (1943).

    CAS  Google Scholar 

  5. Chapman, R.A., and K.M. Mackay, J. Am. Oil Chem. Soc. 26:360 (1949).

    CAS  Google Scholar 

  6. Stine, C.M., H.A. Harland, S.T. Caulter and R. Jeness, J. Dairy Sci. 37:202 (1954).

    Article  CAS  Google Scholar 

  7. Koch, R.B., B. Stern and C.G. Ferrari, Arch. Biochem. Biophys. 78:165 (1958).

    Article  PubMed  CAS  Google Scholar 

  8. Mitsuda, H., K. Yasumoto and K. Iwami, J. Jpn. Soc. Food Nutr. 19:210 (1966).

    CAS  Google Scholar 

  9. Hartman, L., J. Sci. Food Agric. 5:476 (1954).

    Article  CAS  Google Scholar 

  10. Siddiqi, A.M., and A.L. Tappel, Chemist. Analyst 44:52 (1955).

    CAS  Google Scholar 

  11. Hicks, M., and J.M. Gebicki, Anal. Biochem. 99:249 (1979).

    Article  PubMed  CAS  Google Scholar 

  12. Stamm, J., J. Bull. Soc. Pharm. Estonia 5:181 (1925).

    CAS  Google Scholar 

  13. Hamm, D.L., E.G. Hammond, V. Parvanah and H.E. Snyder, J. Am. Oil Chem. Soc. 42:920 (1965).

    CAS  Google Scholar 

  14. Eskin, N.A.M., and C. Frankel, J. Am. Oil Chem. Soc. 53:746 (1976).

    CAS  Google Scholar 

  15. Takagi, T., Y. Mituno and M. Masumura, Lipids 13:147 (1978).

    Article  CAS  Google Scholar 

  16. Asakawa, T., and S. Matsushita, J. Jpn. Soc. Food Nutr. 28:403 (1975).

    CAS  Google Scholar 

  17. Asakawa, T., and S. Matsushita, J. Jpn. Soc. Food Nutr. 29:408 (1976).

    CAS  Google Scholar 

  18. Asakawa, T., and S. Matsushita, Bull. Res. Inst. Food Sci., Kyoto Univ. 40:12 (1977).

    CAS  Google Scholar 

  19. Asakawa, T., and S. Matsushita, J. Am. Oil Chem. Soc. 55:619 (1978).

    CAS  Google Scholar 

  20. Terao, J., T. Ogawa and S. Matsushita, Agric. Biol. Chem. 39:397 (1975).

    CAS  Google Scholar 

  21. Baldwin, R.R., R.S. Bear and R.E. Rundle, J. Am. Chem. Soc. 66:111 (1944).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Institute for Food Science, Kyoto University, 611, Uji, Kyoto, Japan

    T. Asakawa & S. Matsushita

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  1. T. Asakawa
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  2. S. Matsushita
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Asakawa, T., Matsushita, S. A colorimetric microdetermination of peroxide values utilizing aluminum chloride as the catalyst. Lipids 15, 965–967 (1980). https://doi.org/10.1007/BF02534423

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  • DOI: https://doi.org/10.1007/BF02534423

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