Skip to main content
SpringerLink
Log in

A colorimetric assay of pancreatic lipase: Rapid detection of lipase and colipase separated by gel filtration

  • Methods
  • Published:
Lipids

Abstract

A rapid assay for pancreatic lipase (E.C., glycerol-ester hydrolase 3.1.1.3) is described. The assay is based on the color change of a pH indicator as butyric acid is released from the substrate tributyrin. A mixture made with tributyrin and the water soluble components of the assay is ideally suited for use as a rapid test as, for example, when assaying chromatography fractions. Quantitative data can be obtained by measuring the disappearance of absorbance at 557 nm versus a blank reaction. The assay has been used in the rapid preparation of colipase-free lipase and colipase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Verger, R. (1984) in Lipases (Borgstrom, B., and Brockman, H., eds.) pp. 83–150, Elsevier Biomedical, Amsterdam, New York.

    Google Scholar 

  2. Rathelot, J., Julien, R., Canioni, P., Coeroli, C., and Sarda, L. (1975) Biochimie 57, 1117–1122.

    PubMed  CAS  Google Scholar 

  3. Borgstrom, B., and Erlanson, C. (1973) Eur. J. Biochem. 37, 60–68.

    Article  PubMed  CAS  Google Scholar 

  4. Borgstrom, B., and Erlanson, C. (1971) Biochim. Biophys. Acta. 242, 509–513.

    PubMed  CAS  Google Scholar 

  5. Handbook of Chemistry and Physics (1974) (Weast, R., ed.) Vol. 55, p. D-116, CRC Press, Inc.

  6. Patton, J., Donnér, J., and Borgstrom, B. (1978) Biochim. Biophys. Acta 529, 67–78.

    PubMed  CAS  Google Scholar 

  7. Vandermeers, A., Vandermeers-Piret, M., Rathè, J., and Christophe, J. (1974) Biochim. Biophys. Acta 370, 257–268.

    PubMed  CAS  Google Scholar 

  8. Patton, J., Albertsson, P-A., Erlanson, C., and Borgstrom, B. (1978) J. Biol. Chem. 253, 4195–4202.

    PubMed  CAS  Google Scholar 

  9. Verger, R., DeHaas, G., Sarda L., and Desnuelle, P. (1969) Biochim. Biophys. Acta 188, 272–278.

    PubMed  CAS  Google Scholar 

  10. Morgan, R., Barrowman, J., and Borgstrom, B. (1969) Biochim. Biophys. Acta 175, 65–75.

    PubMed  CAS  Google Scholar 

  11. Patton, J., and Andersson, L. (1978) FEBS Lett. 86, 179–181.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, The University of Kansas, 66045, Lawrence, KS

    Johnna Roberts & Valentino J. Stella

  2. Biochemical Research Service Laboratory, The University of Kansas, 66045, Lawrence, KS

    Charles J. Decedue

Authors
  1. Johnna Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Valentino J. Stella
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Charles J. Decedue
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Roberts, J., Stella, V.J. & Decedue, C.J. A colorimetric assay of pancreatic lipase: Rapid detection of lipase and colipase separated by gel filtration. Lipids 20, 42–45 (1985). https://doi.org/10.1007/BF02534361

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02534361

Keywords

Search

Navigation