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The effect of isomerictrans-18∶1 acids on the desaturation of palmitic, linoleic and eicosa-8,11,14-trienoic acids by rat liver microsomes

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Lipids

Abstract

The inhibitory effects of the positional isomers oftrans-18∶1 acids on the desaturation of palmitic acid to palmitoleic (Δ9-desaturase), linoleic to γ-linolenic (Δ6-desaturase) and eicosa-8,11,14-trienoic to arachidonic acid (Δ5-desaturase) were investigated. Thesetrans-18∶1 acids were found to be inhibitory for the microsomal Δ6-, Δ9- and Δ5-desaturases of rat liver. The position of the double bond in thetrans-18∶1 acids seems to be important in determining the degree of inhibition. At inhibitor/substrate ratio of 3∶1, the Δ6-desaturase was most strongly inhibited bytrans-Δ3,-Δ4,-Δ7 and-Δ15-18∶1 isomers, whereas the Δ9-desaturase was most strongly inhibited bytrans-Δ3,-Δ5,-Δ7,-Δ10,-Δ12,-Δ13 and-Δ16 isomers. At inhibitor/substrate ratio of 6∶1, the Δ5-desaturase was most strongly inhibited by Δ3-, Δ9-, Δ13- and Δ15-isomers. When 18∶0 was added to the incubations of 16∶0, 18∶2 and 20∶3 at the same I/S ratios used for thetrans-18∶1 acids, weak inhibition for Δ9-desaturase and no inhibition for Δ5-and Δ6-desaturases was observed.

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Authors and Affiliations

  1. The Hormel Institute, University of Minnesota, 55912, Austin, Minnesota

    M. M. Mahfouz, S. Johnson & R. T. Holman

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  1. M. M. Mahfouz
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  2. S. Johnson
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  3. R. T. Holman
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Mahfouz, M.M., Johnson, S. & Holman, R.T. The effect of isomerictrans-18∶1 acids on the desaturation of palmitic, linoleic and eicosa-8,11,14-trienoic acids by rat liver microsomes. Lipids 15, 100–107 (1980). https://doi.org/10.1007/BF02533884

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  • DOI: https://doi.org/10.1007/BF02533884

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