Abstract
The de novo synthesis of choline and ethanolamine phosphoglycerides in brain microsomes from 18 month-old male rats was investigated in vitro by using labeled cytidine-5′-diphosphate choline and cytidine-5′-diphosphate ethanolamine as lipid precursors. The rate of synthesis of the two phospholipid classes was found to be noticeably decreased, as compared to that of adult animals. The addition of exogenous diacyl glycerols to microsomes from ageing rat brain brings the rate of synthesis nearly to the adult levels. The synthesis of choline and ethanolamine phosphoglycerides is not affected in the liver microsomes of ageing rats. The molar distribution of fatty acids in brain microsomal diacyl glycerols of ageing rats is noticeably different from that of adult animals. The content of monoenoic and dienoic species is increased, whereas that of the tetraenoic species is decreased. Base exchange reaction for choline and ethanolamine incorporation into respective phospholipids is not affected in the brain microsomes of the aged rats.
Similar content being viewed by others
References
“Clinical, Morphological and Neurochemical Aspect in the Ageing Central Nervous System,” Edited by H. Brody, D. Harman, and J. Mark Ordy, Raven Press, New York, 1975.
“Neurobiology of Ageing,” Edited by R.D. Terry, and S. Gershon, Raven Press, New York, 1976.
Herrmann, R.L., in “Handbook of Neurochemistry,” Vol. 5, Pt. B, Edited by A. Lajtha, Plenum Press, New York, 1971, pp. 481–488.
Dayan, A.D., in “Ageing of the Central Nervous System,” Edited by H.M. Van Praag and A.F. Kalverboer, DeErven F. Bohn Publishers, Haarlem, 1972, pp. 58–75.
Bowen, D.M., and A.N. Davison, VIth International Meeting of the Internat. Society for Neurochemistry, Copenhagen, August 21–26, 1977, Commun. No. 146, p. 175.
Rouser, G., and A. Yamamoto, in “Handbook of Neurochemistry,” Vol. 1, Edited by A. Lajtha, Plenum Press, New York, 1969, pp. 121–169.
Rouser, G., C. Galli, and D. Kritchewsky, J. am. Oil Chem. Soc. 42:404 (1965).
Dhopeshwarkar, G.A., and J.F. Mead, in “Clinical, Morphological and Neurochemical Aspects in the Ageing Central Nervous System,” Edited by H. Brody, D. Harman, and J. Mark Ordy, Raven Press, New York, 1975, pp. 119–132.
Horrocks, L.A., G.Y. Sun, and R.A. D'Amato, in “Neurobiology of Ageing,” Edited by J.M. Ordy and K.R. Brizzee, Plenum Press, New York, 1975, pp. 359–367.
“Form and Function of Phospholipids,” Edited by G.B. Ansell, R.M.C. Dawson, and J.N. Hawthorne, Elsevier, Amsterdam, 1973.
Porcellati, G., M. Brunetti, G.E. De Medio, E. Francescangeli, A. Gaiti, and G. Trovarelli, in “Function and Biosynthesis of Lipids,” Edited by R. Brenner, N.G. Bazan, and N. Giusto, Plenum Press, New York, 1977, pp. 476–491.
Porcellati, G., A. Gaiti, H. Woelk, G.E. De Medio, M. Brunetti, E. Francescangeli, and G. Trovarelli, in “Enzymes of Lipid Metabolism,” Edited by S. Gatt, L. Freysz, and P. Mandel, Plenum Press, New York, 1978, pp. 287–299.
Porcellati, G., G. Arienti, M. Pirotta, and D. Giorgini, J. Neurochem. 18:1395 (1971).
Gaiti, A., G. Goracci, G.E. De Medio, and G. Porcellati, FEBS Lett. 27:116 (1972).
Binaglia, L., R. Roberti, G. Michal, and G. Porcellati, Int. J. Biochem. 4:497 (1973).
Gaiti, A., G.E. De Medio, M. Brunetti, L. Amaducci, and G. Porcellati, J. Neurochem. 23:1153 (1974).
Gaiti, A., M. Brunetti, H. Woelk, and G. Porcellati, Lipids 11:823 (1976).
Binaglia, L., G. Goracci, G. Porcellati, R. Roberti, and H. Woelk, J. Neurochem. 21:1067 (1973).
Porcellati, G., M.G. Biasion, and G. Arienti, Lipids 5:725 (1970).
Wieland, O., in “Methods in Enzymatic Analysis,” Edited by H.V. Bergmeyer, Verlag Chemie, Wienheim, 1974, pp. 1404–1408.
Binaglia, L., R. Roberti, and G. Porcellati, in “Enzymes of Lipid Metabolism,” Edited by S. Gatt, L. Freysz, and P. Mandel, Plenum Press, New York, 1978, pp. 353–366.
Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall, J. Biol. Chem. 193:265 (1951).
Porcellati, G., M.G. Biasion, and M. Pirotta, Lipids 5:734 (1970).
De Medio, G.E., D. Giorgini, and G. Porcellati, Farmaco 27:17 (1972).
Giorgini, D., G.E. De Medio, and G. Porcellati, Farmaco 27:3 (1972).
Soto, E.F., J.M. Pasquini, and L. Krawiec, Arch. Biochem. Biophys. 150:362 (1972).
Porcellati, G., in “Advances in Enzyme Regulations,” Vol. X, Edited by G. Weber, Pergamon Press, New York, 1972, pp. 83–100.
Shelanski, M.L., in “Neurobiology of Ageing,” Edited by R.D. Terry and S. Gershon, Raven Press, New York, 1976, pp. 339–349.
Porcellati, G., in “Dietary Lipids and Postnatal Development,” Edited by C. Galli, G. Jacini, and A. Pecile, Raven Press, New York, 1973, pp. 217–222.
De Medio, G.E., H. Woelk, A. Gaiti, F. Frantini, and G. Porcellati, Ital. J. Biochem. 24:335 (1975).
Author information
Authors and Affiliations
About this article
Cite this article
Brunetti, M., Gaiti, A. & Porcellati, G. Synthesis of phosphatidylcholine and phosphatidylethanolamine at different ages in the rat brain in vitro. Lipids 14, 925–931 (1979). https://doi.org/10.1007/BF02533507
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02533507