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Effect of dietary di-2-ethylhexyl phthalate on oxidation of14C-palmitoyl CoA by mitochondria from mammalian heart and liver

  • Published:
Lipids

Abstract

Oxidation of [1-14C] palmitoyl CoA by heart and liver mitochondria from rats fed dietary di-2-ethylhexyl phthalate (DEHP) was investigated in vitro. Oxidation of14C-palmitoyl CoA to14CO2 increased two- to threefold in hepatic mitochondria from rats fed 0.1% DEHP for 2 to 3 days; this increase appeared to be a maximum response since similar data were obtained using hepatic mitochondria from rats receiving 0.5% or 1.0% DEHP in the diet. The response of hepatic mitochondria to DEHP was found to continue throughout the duration of 35-day trials in which 1.0% DEHP was fed. In contrast to hepatic mitochondria, the oxidation of14C-palmitoyl CoA by heart mitochondria decreased ca. 40% upon addition of 0.1% or 0.5% DEHP to the diet; this effect of DEHP on heart mitochondria was not sustained beyond ca. 8 days of DEHP feeding. Limited studies were also performed in rabbits and pigs. Oxidation of14C-palmitoyl CoA was increased ca. twofold in hepatic mitochondria from rabbits fed 1% dietary DEHP for 12 days and in hepatic mitochondria from pigs that received 5 doses of DEHP (0.8g/kg) at 12-hr intervals; the oxidation14C-palmitoyl CoA by heart mitochondria from these same animals was unchanged in the rabbit but increased an average of 37% in the pig. DEHP feeding to rats was associated with increased yields of hepatic mitochondrial protein; standardized preparations of heart mitochondria were not similarly affected.

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Author notes

  1. Frank P. Bell

    Present address: Diabetes-Atherosclerosis Research Unit, The Upjohn Company, 49001, Kalamazoo, MI

Authors and Affiliations

  1. Department of Pathology, McMaster University, Hamilton, Ontario, Canada

    Frank P. Bell & Peter J. Gillies

Authors
  1. Frank P. Bell
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  2. Peter J. Gillies
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Bell, F.P., Gillies, P.J. Effect of dietary di-2-ethylhexyl phthalate on oxidation of14C-palmitoyl CoA by mitochondria from mammalian heart and liver. Lipids 12, 581–585 (1977). https://doi.org/10.1007/BF02533385

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  • DOI: https://doi.org/10.1007/BF02533385

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