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The enzymic synthesis of ganglioside: I. Brain uridine diphopphate D-galactose: N-acetyl-galactosaminyl-galactosyl-glucosyl-ceramide galactosyl transferase

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Lipids

Abstract

An enzyme which catalyzes the transfer of galactose from UDP-galactose to galNAc-gal-glc-ceramide is described. The enzyme is found mainly in the nervous tissue of tadpole (Taylor and Kollros stage 17), adult frog, adult and 8 day old rat. The enzymic activity is localized in the 11,500 xg, 20,000 xg and 100,000 xg particles. The UDP-galactose: galNAc-gal-glc-ceramide from the particles by treatment with sodium desoxylcholate and Triton X-100. The pH optimum for the solubilized enyme is between 6.8 and 7.0 in cacodylate buffer, and the Km is 4.25 ×10−5 M. The enzymic reaction is proportional to time for 4 hr and to the amount of protein added. The product of the transferase reaction, using galNAc-gal-glc-ceramide. A pathway for the biosynthesis of brain gangliosides requiring UDP-galactose: galNAc-gal-glc-ceramide galactosyl transferase is proposed.

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Author notes

  1. Morris C. M. Yip

    Present address: Department of Biological Chemistry, Harvard Medical School and the Cancer Research Institute, New England Deaconess Hospital, 02215, Boston, Mass

Authors and Affiliations

  1. Department of Biochemistry, University of Rhode Island, 02881, Kingston, Rhode Island

    Morris C. M. Yip & Joel A. Dain

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  1. Morris C. M. Yip
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  2. Joel A. Dain
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Previous publications under the name of Morris G. S. Mark.

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Yip, M.C.M., Dain, J.A. The enzymic synthesis of ganglioside: I. Brain uridine diphopphate D-galactose: N-acetyl-galactosaminyl-galactosyl-glucosyl-ceramide galactosyl transferase. Lipids 4, 270–277 (1969). https://doi.org/10.1007/BF02533185

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  • DOI: https://doi.org/10.1007/BF02533185

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