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Inhibition of sterol biosynthesis in chlorella ellipsoidea by AY-99441

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Lipids

Abstract

WhenChlorella ellipsoidea was grown in the presence of 4 ppm AY-9944, complete inhibition of Δ5-sterol biosynthesis was achieved. However total sterol production remained unaltered. As a result a number of sterols accumulated that appear to be intermediates in sterol biosynthesis. These sterols were described and identified as (24S)-5α-ergost-8(9)-en3β-ol, (24S)-5α-stigmast-8(9)-en-3β-ol, 4α-methyl-(24S)-5α-ergosta-8, 14-dien-3β-ol, 4α-methyl-(24S)-5α-stigmasta-8, 14-dien-3β-ol, 4α-methyl-(24S)-5α-ergost-8(9)-en-3β-ol and (24S)-4α-methyl-5α-stigmast-8(9)-en-3β-ol. The occurrence of these sterols inChlorella ellipsoidea is the first time they have been noted in biological material. The accumulation of these sterols in treated cultures indicates that AY-9944 is an extremely effective inhibitor of the Δ8→Δ7 isomerase and the Δ14 reductase of these plants. The occurrence of small amounts of other sterols in treated cultures has led to a proposed pathway for thebiosynthesis of sterols inChlorella ellipsoidea.

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Author notes

  1. Leal G. Dickson

    Present address: Department of Biology, Walla Walla College, College Place, 99324, Wash.

Authors and Affiliations

  1. Department of Botany, University of Maryland, 20742, College Park, Maryland

    Leal G. Dickson & Glenn W. Patterson

Authors
  1. Leal G. Dickson
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  2. Glenn W. Patterson
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Additional information

Scientific Article No. A1775, Contribution No. 4565 of the Maryland Agricultural Experiment Station.

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Dickson, L.G., Patterson, G.W. Inhibition of sterol biosynthesis in chlorella ellipsoidea by AY-99441. Lipids 7, 635–643 (1972). https://doi.org/10.1007/BF02533069

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  • DOI: https://doi.org/10.1007/BF02533069

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