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2-Aminoethylphosphonic acid metabolism in the rat

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Lipids

Abstract

Time course studies of the incorporation of radioactive 2-aminoethylphosphonic acid (AEP) into the tissues of rats demonstrated that maximum incorporation into the liver lipids occurred within 12 to 30 hr after injection, compared to 2 to 3 hr for the incorporation of phosphorylethanolamine. Little incorporation of AEP was observed in the other tissues investigated (heart, lung, spleen, adipose, kidney). The AEP was incorporated to the greatest extent into 1,2-diacylglyceryl-aminoethylphosphonate (diacylglyceryl-AEP), the phosphonate analogue of phosphatidylethanolamine, with some incorporation into the lyso derivative. Diacylglycerol-AEP apparently was not further metabolized by the rat; no methylation of diacylglyceryl-AEP to phosphonolecithin was observed. Subcellular fractionation was performed on the livers of rats who received3H-AEP 12, 30, 36, and 48 hr prior to sacrifice. The greatest amount of radioactivity was recovered in the soluble fractions. Lipid extraction was performed on the subcellular fractions, and most of the radioactivity present in the lipids was found in the microsomal fraction, with the next highest recovery in the mitochondrial and nuclear fractions.

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Authors and Affiliations

  1. Department of Biological Chemistry, University of Illinois at the Medical Center, 60612, Chicago, Illinois

    Jean Curley-Joseph & Thomas O. Henderson

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  1. Jean Curley-Joseph
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  2. Thomas O. Henderson
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Additional information

This work is based upon a thesis submitted by J.C.-J. to the Graduate College of the University of Illinois at the Medical Center in partial fulfillment of the requirements for the Ph.D. degree in Biological Chemistry. Present Address: Department of Biochemistry, Northwestern University Medical Center, Chicago, Illinois, 60611.

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Curley-Joseph, J., Henderson, T.O. 2-Aminoethylphosphonic acid metabolism in the rat. Lipids 12, 75–84 (1977). https://doi.org/10.1007/BF02532976

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  • DOI: https://doi.org/10.1007/BF02532976

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