Abstract
The fate of labeled linoleic, α-linolenic, and higher homologs of α-linolenic acid administered to the yellow clam,Mesodesma mactroides, was investigated. It was found that the clam incorporated the acids dissolved in sea water and converted 18∶2 (n−6) into 20∶2 (n−6) and 18∶3 (n−3) into 18∶4 (n−3) and 20∶3 (n−3). The addition of casein hydrolysate to the sea water increased the desaturation capacity of the clam and allowed the conversion of 18∶2 (n−6) into 18∶3 (n−6) to be demonstrated. An enhanced desaturation of 18∶3 (n−3) into 18∶4 (n−3) was also demonstrated. After 12 hr administration of the acid, no radioactivity was found in arachidonic, 20∶5 (n−3), or 22∶6 (n−3). Feeding the clams a culture ofPhaeodactylum tricornutum previously incubated with 1-14C-α-linolenic acid demonstrated that all the homologs of the α-linolenic series were found in the clam without any important changes. Six hour administration of labeled linolenic acid resulted in the incorporation of the acid into diglycerides and phospholipids.
Similar content being viewed by others
References
Moreno, E.A. de, V.J. Moreno, and R.R. Brenner, Lipids 11:334.
Ackman, R.G., C.S. Tocher, and J. McLachlan, J. Fish. Res. Board Can. 25:1603 (1968).
Chuecas, L., and J.P. Riley, J. Mar. Biol. Assoc. U.K. 49:97 (1969).
Dunham, J., G.H. Harrington, and G. Holz, Jr., Biol. Bull. (Woods Hole, Mass.) 131:389 (1966).
Harrington, G.M., and G. Holz, Jr., Biochim. Biophys. Acta 164:137 (1968).
Folch, J., M. Lees, and G.H. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).
Brenner, R.R., and R.O. Peluffo, Ibid. 241:5213 (1966).
Bray, G.A., Anal. Biochem. 1:279 (1960).
Wren, J.J., J. Chromatogr. 4:173 (1960).
Testerman, J.K., Biol. Bull. (Woods Hole, Mass.) 142:160 (1972).
Moreno, V.J., E.A. de Moreno, and R.R. Brenner, Congr. Arg. Cienc. Biol. Buenos Aires, 1970, p. 161.
Mead, J.F., Fed. Proc. Fed. Am. Soc. Exp. Biol. 20:952 (1961).
Nugteren, D.H., Biochim. Biophys. Acta 60:656 (1962).
Marcel, Y.L., K. Christiansen, and R. Holman, Ibid. 164:25 (1968).
Brenner, R.R., Mol. Cell. Biochem. 3:41 (1974).
Ullman, D., and H. Sprecher, Biochim. Biophys. Acta 248:186 (1971).
Sprecher, H., and Chui-Jong Lee, Ibid. 388:113 (1975).
Ayala, S., and R.R. Brenner, Acta Physiol. Lat. Am. (In press).
Seubert, W., and E.R. Podack, Mol. Cell. Biochem. 1:29 (1973).
Van Der Horst, D.J., Comp. Biochem. Physiol. 46B:551 (1973).
Klenk, E., and H. Mohrhauer, Hoppe-Seyler’s Z. Physiol. Chem. 370:218 (1960).
Kayama, M., Y. Tsuchiya, and J.F. Mead, Bull. Japan Soc. Sci. Fish. 29:452 (1963).
Inkpen, C.A., R.A. Harris, and F.W. Quackenbush, J. Lipid Res. 10:277 (1969).
Gomez Dumm, I.N.T. de, R.O. Peluffo, and R.R. Brenner, Lipids 7:590 (1972).
Kennedy, E.A., Ann. Rev. Biochem. 26:119 (1957).
Bilinsky, E., J. Fish. Res. Board Can. 19:505 (1962).
Shieh, H.S., Comp. Biochem. Physiol. 27:533 (1968).
Shieh, H.S., Ibid. 30:679 (1971).
Author information
Authors and Affiliations
Additional information
Member of the carrera del Investigador Cientifico of the Consejo Nacional de Investigaciones Cientificas y Tecnicas
About this article
Cite this article
De Moreno, J.E.A., Moreno, V.J. & Brenner, R.R. Lipid metabolism of the yellow clam,Mesodesma mactroides: 2-Polyunsaturated fatty acid metabolism. Lipids 11, 561–566 (1976). https://doi.org/10.1007/BF02532902
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02532902