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Incorporation of choline-1,2-14C into molecular species of phosphatidylcholine by alfalfa leaflet tissue

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Lipids

Abstract

Alfalfa leaflet tissue incorporated choline-1,2-14C into at least 6 molecular speies of phosphatidylcholine. After 7 hr incubation the species containing palmitic and linoleic acids (16∶0–18∶2) and the species containing linolenic and linoleic acids (18∶3–18∶2) had the highest specific activities. After 24 hr incubation, the species containing palmitic and linolenic acids (16∶0–18∶3) and the species containing 2 linolenic acids (18∶3–18∶3) had the highest specific activities. The most likely interpretation of these data is that choline is accepted by diglycerides containing one linoleic acid residue and that this residue is then desaturated within the phosphatidylcholine molecule to linolenic acid.

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References

  1. Harris, R.V., and A.T. James, Acta Biochim. Biophys. 106:456 (1965).

    CAS  Google Scholar 

  2. Kanangara, C.G., and P.K. Stumpf, Arch. Biochem. Biophys. 148:414 (1972).

    Article  Google Scholar 

  3. Nagai, J., and K. Bloch, J. Biol. Chem. 241:1925 (1966).

    PubMed  CAS  Google Scholar 

  4. Vijay, J.K., and P.K. Stumpf, Ibid. 246:2910 (1971).

    PubMed  CAS  Google Scholar 

  5. Appleby, R.S., R. Safford and B.W. Nichols, Acta Biochim. Biophys. 248:205 (1971).

    CAS  Google Scholar 

  6. Gurr, M.I., Lipids 6:266 (1971).

    Article  CAS  Google Scholar 

  7. Gurr, M.I., and P. Brawn, Eur. J. Biochem. 17:19 (1970).

    Article  PubMed  CAS  Google Scholar 

  8. Gurr, M.I., M.P. Robinson and A.T. James, Ibid. 9:70 (1969).

    Article  PubMed  CAS  Google Scholar 

  9. Nichols, B.W., Lipids 3:354 (1968).

    Article  CAS  PubMed  Google Scholar 

  10. Nichols, B.W., and R. Moorhouse, Ibid. 4:311 (1969).

    Article  PubMed  CAS  Google Scholar 

  11. Roughan, P.G., Biochem. J. 117:1 (1970).

    PubMed  CAS  Google Scholar 

  12. Williams, J.P., and H.T. Merrilees, Lipids 5:367 (1970).

    Article  PubMed  CAS  Google Scholar 

  13. Gardner, H.W., J. Lipid Res. 9:139 (1968).

    PubMed  CAS  Google Scholar 

  14. Kupier, P.J.C., Plant Physiol. 45:684 (1970).

    Google Scholar 

  15. Arvidson, G.A.E., J. Lipid Res. 6:574 (1965).

    PubMed  CAS  Google Scholar 

  16. Dittmer, J.C., and R.L. Lester, Ibid. 5:126 (1964).

    CAS  Google Scholar 

  17. Bregoff, H.M., E. Roberts and C.C. Delwiche, J. Biol. Chem. 205:565 (1953).

    PubMed  CAS  Google Scholar 

  18. Grenier, G., A. Tremolieres, H.P. Therien and C. Willemot, Can. J. Bot. 50:1681 (1972).

    Article  CAS  Google Scholar 

  19. Bray, G.A., Anal. Biochem. 1:279 (1960).

    Article  CAS  Google Scholar 

  20. Kennedy, E.P., J. Biol. Chem. 209:525 (1954).

    PubMed  CAS  Google Scholar 

  21. Vandor, S.L., and K.E. Richardson, Can. J. Biochem. 46:1309 (1968).

    PubMed  CAS  Google Scholar 

  22. Rytter, D.J., and W.E. Cornatzer, Lipids 7:142 (1972).

    Article  PubMed  CAS  Google Scholar 

  23. Tinoco, J., S.M. Hopkins, D.J. McIntosh, G. Sheehan and R.L. Lyman, Ibid. 2:479 (1967).

    Article  CAS  PubMed  Google Scholar 

  24. Devor, K.S., and J.B. Mudd, J. Lipid Res. 12:403 (1971)

    PubMed  CAS  Google Scholar 

  25. Mudd, J.B., L.M.G. van Golde and L.L.M. van Deenen, Acta Biochim. Biophys. 176:547 (1969).

    CAS  Google Scholar 

  26. Devor, K.A., and J.B. Mudd, J. Lipid Res. 12:412 (1971).

    PubMed  CAS  Google Scholar 

  27. Mudd, J.B., H.H.D.M. van Vliet and L.L.M. van Deenen, Ibid. 10:623 (1969).

    CAS  Google Scholar 

  28. Kates, M., and M. Paradis, Can. J. Biochem. 51:184 (1973).

    Article  PubMed  CAS  Google Scholar 

  29. Hitchcock, C., and B.W. Nichols, in “Plant Lipid Biochemistry,” Academic Press, New York, 1971, p. 145.

    Google Scholar 

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Contribution No. 39, C.D.A. Research Station, Ste-Foy, Quebec.

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Willemot, C., Verret, G. Incorporation of choline-1,2-14C into molecular species of phosphatidylcholine by alfalfa leaflet tissue. Lipids 8, 588–591 (1973). https://doi.org/10.1007/BF02532716

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  • DOI: https://doi.org/10.1007/BF02532716

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