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Studies on the 12α and 26-hydroxylation of bile alcohols by rabbit liver microsomes

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Lipids

Abstract

12α-Hydroxylation of two C27-steroids by rabbit liver microsomes was studied. Optimal assay conditions were determined with 7α-hydroxy-4-cholesten-3-one and 5β-cholestane-3α,7α-diol as substrates. The rate of 12α-hydroxylation of 7α-hydroxy-4-cholesten-3-one was found to be greater than that of 5β-cholestane-3α,7α-diol by ca. 60%. Microsomal 26-hydroxylation of 5β-cholestane-3α7α-diol was also measured, and the ratio of 26-hydroxylation to 12α-hydroxylation of 5β-cholestane-3α,7α-diol was found to be ca. 0.4. Rabbit liver 12-αhydroxylase was more active than that of three other species (man, rat, monkey), explaining in part the predominance of cholic acid in rabbit bile.

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Author notes

  1. Gabriela Nicolau

    Present address: Lederle Laboratories, 10965, Pearl River, NY

Authors and Affiliations

  1. Department of Lipid Research, The Public Health Research Institute of the City of New York, Inc., 10016, New York, New York

    Gabriela Nicolau, Bertram I. Cohen, Gerald Salen & Erwin H. Mosbach

Authors
  1. Gabriela Nicolau
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  2. Bertram I. Cohen
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  3. Gerald Salen
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  4. Erwin H. Mosbach
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Nicolau, G., Cohen, B.I., Salen, G. et al. Studies on the 12α and 26-hydroxylation of bile alcohols by rabbit liver microsomes. Lipids 11, 148–152 (1976). https://doi.org/10.1007/BF02532665

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  • DOI: https://doi.org/10.1007/BF02532665

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