Abstract
Palmitoyl CoA-glycerol-3-phosphate acyltransferase, phosphatidate phosphohydrolase, and phospholipase A were assayed in subcellular fractions of rat lung, including lamellar bodies, the putative site of storage and secretion of lung surfactant. The specific activity of each of these enzymes in lamellar bodies was relatively low and could be entirely accounted for by a small contamination of the lamellar bodies fraction by microsomes, as quantitated by the presence of the microsomal marker reduced triphosphopyridine nucleotide cytochromec reductase. These data indicate that lamellar bodies are not the site of synthesis of the lipid component of pulmonary surfactant by pathways involving these enzymes.
Similar content being viewed by others
References
Goerke, J., Biochim. Biophys. Acta 344:241 (1974).
King, R.J., Fed. Proc. Fed. Amer. Soc. Exp. Biol. 33:2238 (1974).
Kikkawa, Y., K. Yoneda, F. Smith, B. Packard, and K. Suzuki, Lab. Invest. 32:295 (1975).
Page-Roberts, B.A., Biochim. Biophys. Acta 260:334 (1972).
Hoffman, L., J. Cell Physiol. 79:65 (1972).
Gil, J., and O.K. Reiss, J. Cell Biol. 58:152 (1973).
Williams, C.H., Methods in Enzymol. 31:419 (1974).
DiAugustine, R.P., J. Biol. Chem. 249:584 (1974).
Meban, C., J. Cell Biol. 53:249 (1972).
Rooney, S.A., B.A. Page-Roberts, and E.K. Motoyama, Fed. Proc. Fed. Amer. Soc. Exp. Biol. 34:426 (1975).
Engle, M.J., and W.J. Longmore, Ibid. 34:633 (1975).
Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall, J. Biol. Chem. 193:265 (1951).
Ames, B.N., Methods in Enzymol. 8:115 (1966).
Williams, C.H., and H. Kamin, J. Biol. Chem. 237:587 (1962).
Tabor, C.W., H. Tabor, and S.M. Rosenthal, Ibid. 208:645 (1954).
Bligh, E.G., and W.J. Dyer, Can. J. Biochem. Physiol. 37:911 (1959).
Garcia, A., J.D. Newkirk, and R.D. Mavis, Biochem. Biophys. Res. Comm. 64:128 (1975).
Lamb, R.G. and H.J. Fallon, Biochim. Biophys. Acta 238:166 (1974).
Schultz, F.M., J.M. Jimenez, P.C. MacDonald, and J.M. Johnston, Gynecol. Invest. 5:222 (1974).
Akino, T., M. Abe, and T. Arai, Biochim. Biophys. Acta 248:274 (1971).
Vereyken, J.M., A. Montfoort, and L.M.G. van Golde, Ibid. 260:70 (1972).
Kyei-Aboagye, K., D. Rubenstein, and J.C. Beck, Can. J. Biochem. 51:1581 (1973).
Buege, J.A., and S.D. Aust, Biochim. Biophys. Acta 385:371 (1975).
Matsubara, T., R.A. Prough, M.D. Burke, and R.W. Estabrook, Cancer Res. 34:2196 (1974).
Young, S.L., and D.F. Tierney, Amer. J. Physiol. 222:1539 (1972).
Author information
Authors and Affiliations
About this article
Cite this article
Garcia, A., Sener, S.F. & Mavis, R.D. Lung lamellar bodies lack certain key enzymes of phospholipid metabolism. Lipids 11, 109–112 (1976). https://doi.org/10.1007/BF02532659
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02532659