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Phospholipid metabolism in cells in culture

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Lipids

Abstract

The proportions of the different lecithin fractions have been determined in HeLa and KB tissue culture cells and Ehrlich Ascites tumor. 82.8% of the total phosphatidyl choline phosphorus is found in fraction 3 of HeLa cells. The major phosphatidyl cholines found in KB cells and Ehrlich Ascites tumor are in fractions 3 and 4 and representing 66.6% and 88.7% of the total phosphatidyl choline P, respectively. The incorporation of 1,2-14C-choline and 1,2-14C-ethanolamine into the various phosphatidyl choline fractions has been assayed to determine their biosynthesis in Ehrlich Ascites tumor. The incorporation of 1,2-14C-choline into fractions 3 and 4 is 100 times the 1,2-14C-ethanolamine. This evidence indicates that the methylation pathway of phosphatidyl choline synthesis is very low in HeLa, KB and Ehrlich Ascites cells.

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References

  1. Abercrombie, M., and E.J. Ambrose Cancer Res. 22:525 (1962).

    PubMed  CAS  Google Scholar 

  2. Weiss, P., in “International Review of Cytology,” Vol. 7, Edited by G.H. Bourne and J.F. Danielli, Academic Press, New York, 1958, p. 391.

    Google Scholar 

  3. Haven, F.L., and W.R. Bloor, in “Advances in Cancer Research,” Vol. 4, Edited by J.P. Greenstein and A. Haddow, Academic Press, New York, 1956, p. 237.

    Google Scholar 

  4. Swanson, M.A., and C. Artom, J. Biol. Chem. 187:281 (1950).

    PubMed  CAS  Google Scholar 

  5. Spiro, M.J., and J.M. McKibbin, Ibid. 219:643 (1956).

    PubMed  CAS  Google Scholar 

  6. Strickland, E.H., and A.A. Benson, Arch. Biochem. Biophys. 88:344 (1960).

    Article  PubMed  CAS  Google Scholar 

  7. Pfleger, R.C., N.G. Anderson and F. Snyder, Biochemistry 7:2826 (1968).

    Article  PubMed  CAS  Google Scholar 

  8. Ray, T.K., V.P. Skipski, M. Barclay, E. Essner and F.M. Archibald, J. Biol. Chem. 244:5528 (1969).

    PubMed  CAS  Google Scholar 

  9. Johnson, J.D., and W.E. Cornatzer, Proc. Soc. Exp. Biol. Med. 131:474 (1969).

    PubMed  CAS  Google Scholar 

  10. Arvidson, G.A.E., J. Lipid Res. 6:574 (1965).

    PubMed  CAS  Google Scholar 

  11. Kennedy, E.P., and S.B. Weiss, J. Biol. Chem. 222:193 (1956).

    PubMed  CAS  Google Scholar 

  12. Bremer, J., and D.M. Greenberg, Biochim. Biophys. Acta 37:173 (1960).

    Article  PubMed  CAS  Google Scholar 

  13. Rytter, D., J.E. Miller and W.E. Cornatzer, Ibid. 152:418 (1968).

    PubMed  CAS  Google Scholar 

  14. Lee, Y.P., and H.A. Lardy, J. Biol. Chem. 240:1427 (1965).

    PubMed  CAS  Google Scholar 

  15. Folch, J., M. Lees and G.H.S. Stanley, Ibid. 226:497 (1957).

    PubMed  CAS  Google Scholar 

  16. Miller, J.E., and W.E. Cornatzer, Proc. Soc. Exp. Biol. Med. 118:948 (1965).

    PubMed  CAS  Google Scholar 

  17. Fiske, C.H., and Y. Subbarow, J. Biol. Chem. 66:275 (1925).

    Google Scholar 

  18. Parker, F., and N.F. Peterson, J. Lipid Res. 6:455 (1965).

    CAS  Google Scholar 

  19. Tsao, Shuang-Shine, and W.E. Cornatzer, Lipids 2:41 (1967).

    Article  CAS  PubMed  Google Scholar 

  20. Wood, R., and F. Snyder, Arch. of Biochem. Biophys. 131:478 (1969).

    Article  CAS  Google Scholar 

  21. Wood, R., and F. Snyder, Ibid. 131:495 (1969).

    Article  CAS  Google Scholar 

  22. Donisch, V., and R.J. Rossiter, Can. J. Biochem. 44:1461 (1966): Cancer Res. 25: 1463 (1965).

    Article  CAS  Google Scholar 

  23. Figard, P.H., and D.M. Greenberg, Cancer Res. 22:361 (1962).

    PubMed  CAS  Google Scholar 

  24. Baldwin, J.J., and W.E. Cornatzer, Lipids 3:361 (1968).

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Guy and Bertha Ireland Research Laboratory, Department of Biochemistry, University of North Dakota School of Medicine, 58201, Grand Forks, North Dakota

    Darrel J. Rytter & W. E. Cornatzer

Authors
  1. Darrel J. Rytter
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  2. W. E. Cornatzer
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Part of a thesis submitted to the Graduate School of the University of North Dakota in partial fulfillment of the Degree of Master of Science.

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Rytter, D.J., Cornatzer, W.E. Phospholipid metabolism in cells in culture. Lipids 7, 142–145 (1972). https://doi.org/10.1007/BF02532602

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  • DOI: https://doi.org/10.1007/BF02532602

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