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Inhibition of the plasma lecithin-cholesterol acyltransferase reaction by hydrogen peroxide and peroxidized lecithin

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Lipids

Abstract

Inhibition of the plasma lecithincholesterol acyltransferase reaction by hydrogen peroxide and peroxidized lipids was studied by two methods: a radiochemical tracer technique and a direct colorimetric assay. Protection of sulfhydryl groups withp-chloromercuribenzoate, prior to exposure to peroxide, resulted in restoration of acyltransferase activity on removal of the inhibitors with mercaptoethanol. On the basis of these observations, it is proposed that lipid peroxides inhibit the plasma lecithin-cholesterol acyltransferase reaction by alteration of sulfhydryl groups necessary for enzymatic activity.

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Author notes

  1. Hubert S. Mickel

    Present address: Children’s Hospital Medical Center, 02115, Boston, Mass

Authors and Affiliations

  1. Environmental Systems Branch (SMCE), Environmental Sciences Division, USAF School of Aerospace Medicine, Brooks AFB, 78235, Texas

    Hubert S. Mickel, Emmett L. Foulds Jr. &  Dale A. Clark

Authors
  1. Hubert S. Mickel
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  2. Emmett L. Foulds Jr.
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  3. Dale A. Clark
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Mickel, H.S., Foulds, E.L. & Clark, D.A. Inhibition of the plasma lecithin-cholesterol acyltransferase reaction by hydrogen peroxide and peroxidized lecithin. Lipids 7, 121–124 (1972). https://doi.org/10.1007/BF02532599

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  • DOI: https://doi.org/10.1007/BF02532599

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