Abstract
Slices prepared from the mammary glands of lactating mice incorporate only small amounts of (1-14C) acetate, (1-14C) decanoate, or (1-14C) palmitate into lipids. However, when glucose is added to the incubation medium, fatty acid incorporation is stimulated—13-fold from acetate, 17-fold from decanoate, and 2-fold from palmitate. Over 90% of the14C activity in the lipid fraction is in triglycerides. Analysis of fatty acids in the triglycerides showed that almost all of the decanoate and the palmitate were incorporated as intact molecules, while acetate yielded acids of varying chain lengths. The glucose stimulation of triglyceride synthesis is not solely due to its effect upon chain elongation but also could involve glyceride-glycerol availability, as well as other unknown factors. However, from measurements of the amounts of glycerol 3-phosphate in the tissue incubated in the presence of palmitate, it would appear that glyceride-glycerol availability is not rate limiting in triglyceride synthesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Folley, S.J., and T.H. French, Biochem. J. 45:117 (1949).
Folley, S.J., and T.H. French, Ibid. 45:270 (1949).
Folley, S.J., and T.H. French, Ibid. 46:465 (1950).
Balmain, J.H., S.J. Folley, and R.F. Glascock, Ibid. 56:234 (1954).
Hirsch, P.F., H. Baruch, and I.L. Chaikoff, J. Biol. Chem. 210:785 (1954).
Abraham, S., and I.L. Chaikoff, Ibid. 234:2246 (1959).
Abraham, S., P.F. Hirsch, and I.L. Chaikoff, Ibid. 211:31 (1954).
Abraham, S., J. Madsen, and I.L. Chaikoff, Ibid. 239:855 (1964).
McLean, P., Biochim. Biophys. Acta 30:303 (1958).
McLean, P., Biochem. J. 90:271 (1964).
Del Boca, J., and J.P. Flatt, Europ. J. Biochem. 11:127 (1969).
Katz, J., and P.A. Wals, Biochim. Biophys. Acta. 348:344 (1974).
Lossow, W.J., and I.L. Chaikoff, J. Biol. Chem. 230:149 (1958).
Stein, O., and Y. Stein, J. Cell. Biol. 34:251 (1967).
Calvin, M., C. Heidelberger, J.C. Reid, B.M. Tolbert, and P.F. Yankwich, “Isotopic Caron,” John Wiley and Sons, N.Y. 1949, p. 178.
Putman, E.W., and W.Z. Hassid, J. Biol. Chem. 196:749 (1952).
Abraham, S., E.W. Putman, and W.Z. Hassid, Arch. Biochem. Biophys. 41:61 (1952).
Bartley, J.C., H. McGrath, and S. Abraham, Cancer Res. 31:527 (1971).
Johnson, E.J., and S. Abraham, J. Bacteriol. 97:1198 (1969).
Folch, J., M. Lees, and G.H. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).
Johnston, J.M., G.A. Rao, P.A. Lowe, and B.E. Schwarz, Lipids 2:14 (1967).
Abraham, S., K.J. Matthes, and I.L. Chaikoff, Biochim. Biophys. Acta. 49:268 (1961).
“Methods of Enzymatic Analysis” Edited by H.U. Bergmeyer, Academic Press, New York and London, 1965.
Krebs, H.A., and K. Henseleit, Z. Physiol. Chem. 210:33 (1932).
Kinsella, J.E., Int. J. Biochem. 4:549 (1973).
Smith, S., R. Watts, and R. Dils, J. Lipid Res. 9:52 (1968).
Abraham, S., P.F. Kerkoff, and S. Smith, Biochim. Biophys. Acta 261:205 (1972).
Rao, G.A., and S. Abraham, Lipids 8:232 (1973).
Author information
Authors and Affiliations
About this article
Cite this article
Rao, G.A., Abraham, S. Stimulatory effect of glucose upon triglyceride synthesis from acetate, decanoate, and palmitate by mammary gland slices from lactating mice. Lipids 10, 409–412 (1975). https://doi.org/10.1007/BF02532445
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02532445