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Occurrence ofcis-5,cis-9-Hexacosadienoic andcis-5,cis-9,cis-19-Hexacosatrienoic acids in the marine spongeMicrociona prolifera

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Lipids

Abstract

Fatty acid analysis of the total lipids from the marine spongeMicrociona prolifera by gas liquid chromatography on an EGSS-X column revealed two major peaks with equivalent chain length values of 27.08 and 27.74. Each of these components was isolated as a separate band by thin layer chromatography on AgNO3-silicic acid. Characterization of the two unknowns by IR spectroscopy, NMR, hydrogenation, and gas liquid chromatography revealed that the unknown acids weren-26∶2 andn-26∶3 containing only nonmethylene interruptedcis-double bonds. Reductive ozonolysis identified the 26∶2 ascis-5,cis-9-hexacosadienoic acid and the 26∶3 ascis-5,cis-9,cis-19-hexacosatrienoic acid. Analysis of the fatty acid composition ofMicrociona total lipids showed 14% 26∶2 and 31% 36∶3. The neutral lipids, phosphatidylethanomaline, and phosphatidylserine all contained >41% C26 acids; but only 4% C26 was present in the phosphatidylcholine.

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Author notes

  1. Elaine Jefferts

    Present address: Rockefeller University, 10021, New York, N.Y.

Authors and Affiliations

  1. Department of Biochemistry, Nelson Biological Laboratories, Rutgers University, 08903, New Brunswick, New Jersey

    Elaine Jefferts, Reginald W. Morales & Carter Litchfield

Authors
  1. Elaine Jefferts
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  2. Reginald W. Morales
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  3. Carter Litchfield
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Jefferts, E., Morales, R.W. & Litchfield, C. Occurrence ofcis-5,cis-9-Hexacosadienoic andcis-5,cis-9,cis-19-Hexacosatrienoic acids in the marine spongeMicrociona prolifera . Lipids 9, 244–247 (1974). https://doi.org/10.1007/BF02532200

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  • DOI: https://doi.org/10.1007/BF02532200

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