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Determination of plasmalogens after treating with a 2,4-dinitrophenylhydrazine-phosphoric acid reagent

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Lipids

Abstract

A method for the quantitative determination of plasmalogens is described. Glycerophosphatide fractions from animal and plant sources were treated with a 2,4-dinitrophenylhydrazine-phosphoric acid reagent. The plasmalogen content was estimated by two different procedures after a single treatment with reagent: a) by determining the phosphorus content of diacyl phospholipid and (2-acyl) lysophospholipid separated by thin-layer chromatography, b) by spectrophotometric determination of the 2,4-dinitrophenylhydrazones and phosphorus assay of the original untreated sample. The total plasmalogen content of an unfractionated extract may be determined easily by the latter procedure.

The method described gives results comparable with those from the p-nitrophenylhydrazine method and iodination procedure. The usefulness of this 2,4-dinitrophenylhydrazine-phosphoric acid treatment for further structural analyses of plasmalogens is discussed.

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References

  1. Bligh, E. G., and W. J. Dyer, Can. J. Biochem. Physiol.37, 911 (1959).

    PubMed  CAS  Google Scholar 

  2. Dawson, R. M. C., Biochem. J.75, 45 (1960).

    PubMed  CAS  Google Scholar 

  3. Dawson, R. M. C., N. Hemington and J. B. Davenport, Biochem. J.84, 497 (1962).

    PubMed  CAS  Google Scholar 

  4. Dittmer, J. C., and R. L. Lester, J. Lipid Res.5, 126 (1964).

    CAS  Google Scholar 

  5. Feulgen, R., W. Boguth and G. Anderson, Z. Physiol. Chem.287, 90 (1951).

    CAS  Google Scholar 

  6. Folch, J., M. Lees and G. H. Sloane-Stanley, J. Biol. Chem.226, 497 (1957).

    PubMed  CAS  Google Scholar 

  7. Gottfried, E. L., and M. M. Rapport, J. Biol. Chem.237, 329 (1962).

    PubMed  CAS  Google Scholar 

  8. Gray, G. M., Biochem. J.70, 427 (1958).

    Google Scholar 

  9. Jones, L. A., J. C. Holmes and R. B. Seligman, Anal. Chem.28, 191 (1956).

    Article  CAS  Google Scholar 

  10. Katz, I., and M. Keeney, J. Lipid Res.7, 170 (1966).

    PubMed  CAS  Google Scholar 

  11. Klenk, E., and H. Debuch, “Progress in the Chemistry of Fats and Other Lipids”, Vol.6, MacMillian, New York (1963).

    Google Scholar 

  12. Kuchmak, M., and L. R. Dugan, Jr., JAOCS,40, 734 (1963).

    CAS  Google Scholar 

  13. Leupold, F., H. Büttner and K. Ranniger, Z. Physiol. Chem.294, 107 (1954).

    CAS  Google Scholar 

  14. Mookherjee, B. D., and S. S. Chang, JAOCS40, 232 (1963).

    CAS  Google Scholar 

  15. Norton, W. T., Biochem. Biophys. Acta38, 340 (1960).

    Article  PubMed  CAS  Google Scholar 

  16. Owens, K., Biochem. J.100, 354 (1966).

    PubMed  CAS  Google Scholar 

  17. Parker, F., J. Lipid Res.,6, 455 (1965).

    CAS  Google Scholar 

  18. Parks, O. W., M. Keeney and D. P. Schwartz, J. Dairy Sci.46, 295 (1963).

    Article  CAS  Google Scholar 

  19. Pries, C., and C. J. F. Böttcher, Biochem. Biophys. Acta98, 329 (1965).

    PubMed  CAS  Google Scholar 

  20. Rapport, M. M., and N. F. Alonzo, J. Biol. Chem.217, 199 (1955).

    PubMed  CAS  Google Scholar 

  21. Rapport, M. M., and N. F. Alonzo, J. Biol. Chem.235, 1953 (1960).

    PubMed  CAS  Google Scholar 

  22. Rhee, K. S., and L. R. Dugan Jr., Anal. Biochem.19, 157 (1967).

    Article  PubMed  CAS  Google Scholar 

  23. Rouser, G., G. Kritchevsky, D. Heller and E. Lieber, JAOCS40, 425 (1963).

    Google Scholar 

  24. Rouser, G., C. Galli, E. Lieber, M. L. Blank and O. S. Privett, JAOCS41, 836 (1964).

    CAS  Google Scholar 

  25. Rouser, G., A. N. Siakotos and S. Fleischer, Lipids1, 85 (1966).

    CAS  PubMed  Google Scholar 

  26. Schwartz, D. P., and O. W. Parks, Anal. Chem.33, 1396 (1961).

    Article  CAS  Google Scholar 

  27. Schwartz, D. P., H. S. Haller and M. Keeney, Anal. Chem.35, 2191 (1963).

    Article  CAS  Google Scholar 

  28. Thiele, O. W., and H. Bergmann, Z. Physiol. Chem.306, 185 (1957).

    CAS  Google Scholar 

  29. Warner, H. R., and W. E. M. Lands, J. Lipid Res.4, 216 (1963).

    PubMed  CAS  Google Scholar 

  30. Williams, J. N. Jr., C. E. Anderson and A. D. Jasik, J. Lipid Res.3, 378 (1962).

    CAS  Google Scholar 

  31. Wittenberg, J. B., S. R. Korey and F. H. Swenson, J. Biol. Chem.219, 39 (1956).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science, Michigan State University, East Lansing, Michigan

    Ki Soon Rhee, R. R. Del Rosario & L. R. Dugan Jr.

Authors
  1. Ki Soon Rhee
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  2. R. R. Del Rosario
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  3. L. R. Dugan Jr.
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Additional information

Michigan Agr. Expt. Sta. Journal Article No. 3912.

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Rhee, K.S., Del Rosario, R.R. & Dugan, L.R. Determination of plasmalogens after treating with a 2,4-dinitrophenylhydrazine-phosphoric acid reagent. Lipids 2, 334–338 (1967). https://doi.org/10.1007/BF02532121

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  • DOI: https://doi.org/10.1007/BF02532121

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