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Characterizing Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family

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Inositol Phosphates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2091))

Abstract

The diphosphoinositol polyphosphate phosphohydrolases are a subset of the Nudix hydrolase family of enzymes. As such, they metabolize a wide range of substrates, including diphosphoinositol polyphosphates (inositol diphosphates, inositol pyrophosphates), dinucleotide phosphates, nucleosides as well as 5-phosphoribosyl 1-pyrophosphate and inorganic polyphosphate. Here, we describe protocols to optimize these enzymes, with consideration to buffer composition and sample preparation and how to analyze the metabolism of these substrates using high-performance liquid chromatography, giving advice where pitfalls are commonly encountered.

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Author information

Authors and Affiliations

  1. Division of Cell Signalling, School of Life Sciences, University of Dundee, Dundee, UK

    Lucinda Winward

  2. Department of Pharmacy, University of Wolverhampton, Wolverhampton, UK

    Rajagopal Sharada Kilari

  3. RCSI-Bahrain, Adliya, Bahrain

    Stephen T. Safrany

Authors
  1. Lucinda Winward
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  2. Rajagopal Sharada Kilari
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  3. Stephen T. Safrany
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Corresponding author

Correspondence to Stephen T. Safrany .

Editor information

Editors and Affiliations

  1. Department of Chemistry, The Catholic University of America, Washington, DC, USA

    Gregory J. Miller

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Winward, L., Kilari, R.S., Safrany, S.T. (2020). Characterizing Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family. In: Miller, G. (eds) Inositol Phosphates. Methods in Molecular Biology, vol 2091. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0167-9_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0167-9_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0166-2

  • Online ISBN: 978-1-0716-0167-9

  • eBook Packages: Springer Protocols

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