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Biosynthesis of monoacyl-sn-glycerol 3-phosphate by rabbit heart mitochondria: Positional specificity differing from liver enzyme

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Lipids

Abstract

Rabbit heart mitochondria synthesize comparably more monoacyl-than diacylglycerol 3-phosphate as compared to heart microsomes. Since the fatty acid specificity of the biosynthesis by cardiac enzymes differs from that by hepatic enzymes, the positional specificity of monoacylglycerol 3-phosphate formation by mitochondria was investigated by using a method utilizing lysosomal phosphatidate phosphohydrolase and borate-impregnated thin layer chromatography. Contrary to the results of studies with liver particulates, ca. one-third of palmitate was found at position 2 of the glycerol moiety and the remainder at position 1, whereas one-third of oleate was bound at position 1. Only a slight, asymmetric distribution was observed with linoleate as the acyl donor. Thus, our study indicates that preferential acylation of individual fatty acids during the formation of monoacylglycerol 3-phosphate is not as distinct in the heart mitochondria as in the liver mitochondria and microsomes.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, University of Ottawa, K1N 6N5, Ottawa, Ontario, Canada

    M. S. Liu, P. J. Brooks & K. Joe Kako

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  1. M. S. Liu
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  2. P. J. Brooks
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  3. K. Joe Kako
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Liu, M.S., Brooks, P.J. & Kako, K.J. Biosynthesis of monoacyl-sn-glycerol 3-phosphate by rabbit heart mitochondria: Positional specificity differing from liver enzyme. Lipids 9, 391–396 (1974). https://doi.org/10.1007/BF02532056

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  • DOI: https://doi.org/10.1007/BF02532056

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