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Phospholipids of the sea anemone: Quantitative distribution; absence of carbon-phosphorus linkages in glycerol phospholipids; structural elucidation of ceramide aminoethylphosphonate

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Lipids

Abstract

The phospholipid composition of the sea anemone (Anthopleura elegantissima) was determined by quantitative thin-layer chromatography (TLC). Phosphonic acids were not detected in phosphatidyl choline, phosphatidyl ethanolamine, or phosphatidyl serine following isolation and hydrolysis. The structure of ceramide aminoethylphosphonate, a phosphonolipid, was elucidated by hydrolysis and oxidative degradation followed by characterization of the products by TLC and gas-liquid chromatography. The long-chain base was shown to be sphingosine with the 2-aminoethylphosphonic acid group attached through the primary hydroxyl group at carbon one. Ceramide aminoethylphosphonate is therefore 1-(O-phosphonoethylamine)-N-acyl sphingosine.

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Author notes

  1. Gerald Simon

    Present address: Departments of Neurology and Biochemistry, University of Illinois College of Medicine, Presbyterian-St. Luke's Hospital, Chicago, Illinois

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  1. Department of Biochemistry, City of Hope Medical Center, Duarte, California

    Gerald Simon & George Rouser

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  1. Gerald Simon
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  2. George Rouser
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Simon, G., Rouser, G. Phospholipids of the sea anemone: Quantitative distribution; absence of carbon-phosphorus linkages in glycerol phospholipids; structural elucidation of ceramide aminoethylphosphonate. Lipids 2, 55–59 (1967). https://doi.org/10.1007/BF02532001

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  • DOI: https://doi.org/10.1007/BF02532001

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