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Lipids of cultured hepatoma cells: II. Effect of media lipids on cellular phospholipids

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Lipids

Abstract

Minimal deviation hepatoma cells were cultured in a modified Swim's 77 medium supplemented with decreasing amounts of serum, lipid-free serum, and lipid-free serum containing added palmitic or linoleic acids. Cellular phospholipids were extracted and the class distribution determined quantitatively. The fatty acid composition of each phospholipid class was determined, and the percentages from cells grown on each of the various media were compared. Cellular phospholipid class and fatty acid compositions differed from media compositions, indicating that intact serum phospholipids are not incorporated into cellular structures. Phosphatidylcholine percentages decreased as the media serum and lipid levels decreased, while phosphatidylinositol and phosphatidylethanolamine percentages increased. Sphingomyelin of cells grown in medium containing added linoleic acids contained a high level of a 24∶2 acid. All classes, except sphingomyelin, contained elevated levels of 18∶1 acid and decreased levels of polyunsaturated fatty acids, relative to normal rat liver. Cells cultured on lipid-free medium did not contain increased concentrations of 20∶3 acid, suggesting that this hepatoma cell cannot desaturate monoenoic acids. Phosphoglycerides of cells, grown on lipid-free medium, had the highest monoene fatty acid concentration, whereas those cells grown on media containing added linoleic acid had the lowest concentrations, suggesting that linoleate may inhibit or regulate monoenoic acid biosynthesis in this cell. These mass data also demonstrate that monoenoic fatty acid biosynthesis in this cultured hepatoma cell responds to dietary changes.

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References

  1. Wood, R., Lipids 8:690 (1973).

    PubMed  CAS  Google Scholar 

  2. Rouser, G., A.M. Siakotos and S. Fleischer, Ibid. 1:85 (1966).

    Article  CAS  Google Scholar 

  3. Dittmer, J.C., and R.L. Lester, J. Lipid Res. 5:126 (1964).

    CAS  Google Scholar 

  4. Wood, R., Lipids 7:596 (1972).

    Article  PubMed  CAS  Google Scholar 

  5. Rouser, G., G.J. Nelson, S. Fleischer and G. Simon, in “Biological Membranes,” Edited by D. Chapman, Academic Press, New York, 1968, p. 5.

    Google Scholar 

  6. Wood, R., and R.D. Harlow, Arch. Biochem. Biophys. 135:272 (1969).

    Article  PubMed  CAS  Google Scholar 

  7. Bergelson, L.D., E.V. Dyatlovitskaya, T.I. Torkhovskaya, I.B. Sorokina and N.P. Gorkova, Biochim. Biophys. Acta. 210:287 (1970).

    PubMed  CAS  Google Scholar 

  8. Selkirk, J.K., J.C. Elwood and H.P. Morris, Cancer Res. 31:27 (1971).

    PubMed  CAS  Google Scholar 

  9. Wood, R., and R.D. Harlow, Arch. Biochem. Biophys. 131:494 (1969).

    Google Scholar 

  10. Kuksis, A., L. Marai, W.C. Breckenridge, D.A. Gornall and O. Stachnyk, Can. J. Physiol. Pharmacol. 46:511 (1968).

    PubMed  CAS  Google Scholar 

  11. Getz, G.S., W. Bartley, F. Stirpe, B.M. Nolton and A. Renshaw, Biochem. J. 80:176 (1961).

    PubMed  CAS  Google Scholar 

  12. Dittmer, J.C., and D.J. Hanahan, J. Biol. Chem. 234:1976 (1959).

    PubMed  CAS  Google Scholar 

  13. Ruggieri, S., and A. Fallani, in “Tumor Lipids: Biochemistry and Metabolism,” Edited by R. Wood, American Oil Chemists' Society, Champaign, Ill., 1973, p. 89.

    Google Scholar 

  14. Bergelson, L.D., and E.V. Dyatlovitskaya, Ibid. in “, p. 111.

    Google Scholar 

  15. van Hoeven, R.P., and P. Emmelot, Ibid. in “, p. 126.

    Google Scholar 

  16. Ruggieri, S., and A. Fallani, Lo Sperimentale 118:483 (1968).

    PubMed  CAS  Google Scholar 

  17. Wood, R., and R.D. Harlow, Arch. Biochem. Biophys. 135:272 (1969).

    Article  PubMed  CAS  Google Scholar 

  18. Wood, R., and R.D. Harlow, Ibid. 141:183 (1970).

    Article  PubMed  CAS  Google Scholar 

  19. Bailey, J.M., B.V. Howard, L.M. Dunbar and S.F. Tillman, Lipids 7:125 (1972).

    Article  PubMed  CAS  Google Scholar 

  20. Wiegand, R.D., and R. Wood, In press.

  21. Allmann, D.W., and D.M. Gibson, J. Lipid Res. 6:51 (1965).

    PubMed  CAS  Google Scholar 

  22. Abraham, S., Amer. J. Clin. Nutr. 23:1120 (1970).

    PubMed  CAS  Google Scholar 

  23. Bartley, J.C., and S. Abraham, Biochim. Biophys. Acta 280:258 (1972).

    PubMed  CAS  Google Scholar 

  24. Rothblat, G.H., Advan. Lipid Res. 7:135 (1969).

    CAS  Google Scholar 

  25. Jacobs, R.A., W.S. Sly and P.W. Majerus, J. Biol. Chem. 248:1268 (1973).

    PubMed  CAS  Google Scholar 

  26. Sabine, J.R., S. Abraham and H.P. Morris, Cancer Res. 28:46 (1968).

    PubMed  CAS  Google Scholar 

  27. Sabine, J.R., S. Abraham and I.L. Chaikoff, Biochim. Biophys. Acta 116:407 (1966).

    PubMed  CAS  Google Scholar 

  28. Sabine, J.R., S. Abraham and I.L. Chaikoff, Cancer Res. 27:793 (1967).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine and Biochemistry, University of Missouri School of Medicine, 65201, Columbia, Missouri

    Randall Wood & John Falch

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  1. Randall Wood
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  2. John Falch
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Wood, R., Falch, J. Lipids of cultured hepatoma cells: II. Effect of media lipids on cellular phospholipids. Lipids 8, 702–710 (1973). https://doi.org/10.1007/BF02531836

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  • DOI: https://doi.org/10.1007/BF02531836

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