Abstract
The incorporation of inorganic phosphate (as NaH2PO4) into the phospholipids of sheep red blood cells was studied in vitro in blood samples from five highpotassium (HK) and five low-potassium (LK) sheep. The erythrocytes from HK sheep incorporated more activity in 4 hr than those from the LK sheep. However no activity was incorporated into the major phospholipids of the cells (phosphatidyl ethanolamine, phosphatidyl serine, and sphingomyelin) of either group. The phosphatidic acid fraction was labeled in both groups and to a significantly greater extent in the HK samples. However the highest activity in the phospholipid of sheep red-cells was located in three unknown compounds not previously detected. Their specific activities were the same in the HK and the LK samples although they were present in slightly larger amounts in the HK samples. In general, incorporation was at a rather low level, and from stoichiometric considerations it was concluded that the metabolism in the red-cell phospholipids could not be directly involved in the active transport of ions across the cell membrane. This work also confirmed a previous report that no quantitative differences exist among the major phospholipid classes in the two types of cells.
Similar content being viewed by others
References
Marks, P. A., A. Gelhorn and C. Kidson, J. Biol. Chem.235, 2579–2583 (1960).
Josson, L., F. Steven and R. Ruyssen, Bull. Chim. Farm.101, 259–269 (1962).
Raderecht, H. J., S. Binnewies and E. Schölzel, Acta Biol. Med. Ger.8, 199–208 (1962).
Paysant, M., B. Maupin and J. Polonovski, Bull. Soc. Chim. Biol.45, 247–252 (1963).
Sloviter, H. A., and R. K. Bose, Biochim. Biophys. Acta116, 156–158 (1968).
Reed, C. F., J. Clin. Invest.38, 1032–1033 (1959).
Polonovski, J., and M. Paysant, Bull. Soc. Chim. Biol.45, 339–347 (1963).
Sakagami, T., O. Minari and T. Orii, Biochim. Biophys. Acta98, 111–116 (1965).
Sakagami, T., O. Minari and T. Orii, Ibid.98, 356–364 (1965).
Agren, G., and G. Ronquist, Acta Chem. Scand.18, 2029–2043 (1964).
Hokin, L. E., and M. R. Hokin, Biochim. Biophys. Acta84, 563–575 (1964).
Westerman, M. P., and W. N. Jensen, Proc. Soc. Expt. Biol. Med.118, 315–319 (1965).
Jacobs, H. S., and M. Karnovsky, J. Clin. Invest.46, 173–185 (1967).
Bartles, J. F., Ibid.30, 816–824 (1957).
Jacobs, H. S., and J. H. Handl Ibid.43, 1704–1720 (1964).
Evans, J. V., Nature174, 931–932 (1954).
Evans, J. V., and M. S. Mounib, J. Agricultural Sci.48, 433–437 (1957).
Tosteson, D. C., and J. F. Hofman, J. Gen. Physiol.44, 169–194 (1960).
Nelson, G. J., Lipids2, 64–71 (1967).
Snyder, F., “Advances in Tracer Methodology,” Vol. 2, edited by S. Rothschild, Plenum Press, New York, 1965, p. 107.
Nelson, G. J., J. Lipid Res.8, 374–379 (1967).
Kaiser, I. H., and J. N. Cummings, Am. J. Physiol.193, 627–633 (1958).
Nelson, G. J., Lipids2, 323–328 (1967).
Nelson, G. J., Biochim. Biophys. Acta144, 221–232 (1967).
Rouser, G., G. Kritchevsky, A. N. Siakotos and A. Yamamoto, “An Introduction to Neuropathology: Method and Diagnosis”, edited by C. G. Tedeschi, Little Brown and Company, Boston, in press.
Dodge, J. T., and G. B. Phillips, J. Lipid Res.7, 387–395 (1968).
Siakotos, A. N., and G. Rouser, JAOCS42, 913–919 (1965).
Rouser, G., G. Kritchevsky, G. Simon and G. Nelson, Lipids2, 37–40 (1967).
Rouser, G., G. Kritchevsky, D. Heller and E. Lieber, JAOCS40, 425–454 (1963).
Rouser, G., C. Galli, E. Lieber, M. L. Blank and O. S. Privett, Ibid.41, 836–840 (1964).
Rouser, G., A. N. Siakotos and S. Fleischer, Lipids1, 85–86 (1968).
Bartlett, G. R., J. Biol. Chem.234, 466–468 (1959).
Nelson, G. J., and R. A. Booth, Anal. Biochem.20, 198–199 (1967).
Folch, J., M. Lees and G. H. Sloane-Stanley, J. Biol. Chem.226, 497–509 (1957).
Oliveira, M. M., and M. Vaughan, J. Lipid Res.5, 156–162 (1964).
Mulder, E., J. de Gier and L. L. M. van Deenen, Biochim. Biophys. Acta70 94–96 (1963).
Mulder, E., and L. L. M. van Deenen, Ibid.106, 348–356 (1965).
Vandenheuvel, F. A., JAOCS42, 481–492 (1965).
De Graeff, J., E. F. Dempsey, L. D. F. Lameyer and A. Leaf, Biochim. Biophys. Acta106, 155–170 (1965).
Hokin, L. E., and M. R. Hokin, Fed. Proc.22, 8–18 (1963).
Glynn, I. M., C. W. Slayman, J. Eichberg and R. M. C. Dawson, Biochem. J.94, 692–699 (1965).
Tosteson, D. C., Fed. Proc.22, 19–26 (1963).
Tosteson, D. C., P. Cook and R. Blount, J. Gen. Physiol.48, 1125–1143 (1965).
Ronquist, G., and G. Ågren, Nature205, 1021–1022 (1965).
Ronquist, G., and G. Ågren, Ibid.,209, 1090–1091 (1968).
Author information
Authors and Affiliations
About this article
Cite this article
Nelson, G.J. Studies on the lipids of sheep red blood cells. II. The incorporation of phosphorus into phospholipids of HK and LK cells. Lipids 3, 267–274 (1968). https://doi.org/10.1007/BF02531200
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02531200