Abstract
The combustion method has been modified to increase the recovery of tritiated water after combustion of a tritium-labeled tissue sample. This was accomplished by cooling the bottom of the combustion flask in a dry ice-acetone bath while irradiating the top with an infrared lamp. The procedure resulted in at least 92% to 102% recovery of the tritiated water. The NCS solubilizer was found to be superior to hyamine for solubilizing14C labeled tissue samples. The samples yielded light yellow-colored solutions when incubated for 15 hr at 50–55C. The counting efficiency of this solution was 75% or higher.
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Parekh, C.K., Eigen, E. Tritium and14C counting in tissue samples by using liquid scintillation method. Lipids 3, 225–227 (1968). https://doi.org/10.1007/BF02531191
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DOI: https://doi.org/10.1007/BF02531191