Skip to main content
SpringerLink
Log in

A comparative study of gangliosides from the brains of various species

  • Published:
Lipids

Abstract

A comparison is made of the sialic acid and hexose content of crude gangliosides from the brains of 13 species, including mammals, a bird, and amphibian, a reptile and fish. The sialic acid content is relatively constant from species to species. Gangliosides are less abundant in tissues other than the brain. Four species (cat, dog, pig and sheep) were selected for the determination of their major ganglioside subfractions. The ganglioside subfractions were isolated (after extraction and partition dialysis) using descending thin layer chromatography. The population of ganglioside molecules varied from species to species. From dog and sheep a mono-, a di- and a trisialoganglioside were obtained; from cat a mono-, a di- and two trisialogangliosides; and from pig a mono- and two disialogangliosides. Each ganglioside subfraction was found to contain glucose, galactose and galactosamine in the ratio of approximately 1∶2∶1. The fatty acid moieties consisted of more than 80% stearic acid with lesser amounts of arachidic, palmitic and behenic acid. Sphingosine analyses indicated ratios of sphingosine to icosisphingosine of 7∶3 for the monosialo-, 1∶1 for the disialo- and 3∶7 for the trisialogangliosides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Folch-Pi, J., S. Arsove and J. A. Meath, J. Biol. Chem.191, 819–831 (1951).

    CAS  Google Scholar 

  2. Garrigan, O. W., and E. Chargaff, Biochim. Biophys. Acta70, 452–464 (1963).

    Article  PubMed  CAS  Google Scholar 

  3. Privett, O. S., M. L. Blank, D. W. Codding and E. C. Nickell, JAOCS42, 381–393 (1965).

    PubMed  CAS  Google Scholar 

  4. Kuhn, R., and H. Wiegandt, Chem. Ber.96, 866–880 (1963).

    CAS  Google Scholar 

  5. Krishnan, R. S., L. A. Witting and M. R. Horwitt, Federation Proc.25, 2250 (1966).

    Google Scholar 

  6. Svennerholm, L., Biochim. Biophys. Acta24, 604–611 (1957).

    Article  PubMed  CAS  Google Scholar 

  7. Ledeen, R., JAOCS43, 57–66 (1966).

    PubMed  CAS  Google Scholar 

  8. Werner, I., and L. Odin, Acta Soc. Med. Upsalien57, 230–241 (1952).

    CAS  Google Scholar 

  9. Ledeen, R., and K. Salsman, Biochemistry4, 2225–2233 (1965).

    Article  CAS  Google Scholar 

  10. Trevelyan, W. E., and J. S. Harrison, Biochem. J.50, 298–303 (1952).

    PubMed  CAS  Google Scholar 

  11. Partridge, S. M., Nature164, 443 (1949).

    CAS  Google Scholar 

  12. Rosenberg, A., and E. Chargaff, J. Biol. Chem.232, 1031–1049 (1958).

    PubMed  CAS  Google Scholar 

  13. Elson, L. A., and W. T. J. Morgan, Biochem. J.27, 1824–1828 (1933).

    PubMed  CAS  Google Scholar 

  14. Stoffyn, P. J., and R. W. Jeanloz, Arch. Biochem. Biophys.52, 373–379 (1954).

    Article  CAS  Google Scholar 

  15. Sweeley, C. C., and E. A. Moscatelli, J. Lipid Res.1, 40–47 (1959).

    CAS  Google Scholar 

  16. Rosenberg, A., and N. Stern, Ibid.7, 122–131 (1966).

    PubMed  CAS  Google Scholar 

  17. Lemieux, R. U., and E. Von Rudloff, Can. J. Chem.33, 1701–1709 (1955).

    Article  CAS  Google Scholar 

  18. Morrison, W. R., and L. M. Smith, J. Lipid Res.5, 600–608 (1964).

    PubMed  CAS  Google Scholar 

  19. Walz, E., Z. Physiol. Chem.166, 210–222 (1927).

    CAS  Google Scholar 

  20. Levene, P. A., and K. Landsteiner, J. Biol. Chem.75, 607–612 (1927).

    CAS  Google Scholar 

  21. Warren, L., Ibid.234, 1971–1975 (1959).

    PubMed  CAS  Google Scholar 

  22. McIlwain, H., Ann. Rep. Progr. Chem. (Chem. Soc. London)57, 367–379 (1960).

    Google Scholar 

  23. Lehninger, A. L., Proc. Nat. Acad. Sci. U.S.60, 1069–1080 (1968).

    Article  CAS  Google Scholar 

  24. Yiamouyiannis, J. A., and J. A. Dain, Lipids3, 378–379 (1968).

    Article  CAS  Google Scholar 

  25. Kuhn, R., H. Wiegandt and H. Egge, Angew. Chem.73, 580–581 (1961).

    CAS  Google Scholar 

  26. Johnson, G. A., and R. H. McCluer, Biochim. Biophys. Acta70, 487–489 (1963).

    Article  PubMed  CAS  Google Scholar 

  27. Rathnam, P., Ph. D. dissertation, Seton Hall University (1966).

  28. Kishimoto, Y., and N. S. Radin, J. Lipid Res.7, 141–145 (1966).

    PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. C. -L. Schengrund

    Present address: Department of Biological Chemistry, College of Medicine, Pennsylvania State University, 17033, Hershey, Penn

Authors and Affiliations

  1. Department of Chemistry, Seton Hall University, 07079, South Orange, New Jersey

    C. -L. Schengrund & O. W. Garrigan

Authors
  1. C. -L. Schengrund
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. W. Garrigan
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Schengrund, C.L., Garrigan, O.W. A comparative study of gangliosides from the brains of various species. Lipids 4, 488–495 (1969). https://doi.org/10.1007/BF02531030

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02531030

Keywords

Search

Navigation