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Effects of dietary triacylglycerol structure on triacylglycerols of resultant chylomicrons from fish oil- and seal oil-fed rats

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Lipids

Abstract

We investigated the influence of the intramolecular fatty acid distribution of dietary triacyl-sn-glycerols (TAG) rich in n-3 polyunsaturated fatty acids (PUFA) on the structure of chylomicron TAG. Fish oil and seal oil, comparable in fatty acid compositions but with different contents of major n-3 PUFA esterified at thesn-2 position (20:5n-3, 46.6%, and 5.3%; 22:6n-3, 75.5%, and 3.8%, respectively), were fed to rats. Mesenteric lymph was collected and the chylomicrons were isolated by ultracentrifugation. The fatty acid composition of chylomicrons largely reflected the fatty acid composition of the oils administered. The intramolecular fatty acid distributions of the TAG fed were reflected in the chylomicron TAG as the fraction of the total contents observed in thesn-2 position of 20:5n-3 were 23.6 and 13.3%, and of 22:6n-3 were 30.6 and 5.4% for resultant chylomicrons following fish oil and seal oil administration, respectively. Thus, after seal oil administration, significant higher load of n-3 PUFA was esterified in thesn-1,3 positions of chylomicron TAG compared with fish oil administration (P<0.05).

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Abbreviations

MAG:

monoacyl-sn-glycerols

PUFA:

polyunsaturated fatty acids

TAG:

triacyl-sn-glycerol

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Authors and Affiliations

  1. Center for Food Research, Technical University of Denmark, DK-2800, Lyngby, Denmark

    Michael Søberg Christensen & Carl-Erik Høy

  2. Department of Biochemistry and Nutrition, Technical University of Denmark, Building 224, DK-2800, Lyngby, Denmark

    Michael Søberg Christensen & Carl-Erik Høy

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  1. Michael Søberg Christensen
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  2. Carl-Erik Høy
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Christensen, M.S., Høy, CE. Effects of dietary triacylglycerol structure on triacylglycerols of resultant chylomicrons from fish oil- and seal oil-fed rats. Lipids 31, 341–344 (1996). https://doi.org/10.1007/BF02529882

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