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The effects of dietary phospholipids enriched with phosphatidylethanolamine on bile and red cell membrane lipids in humans

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Lipids

Abstract

The role of phospholipids in biliary cholesterol solubilization and crystallization has only recently begun to be appreciated. Phospholipid vesicles are believed to be the metastable carrier from which cholesterol nucleates. Cholesterol crystallization is influenced by the phospholipid species in bile. Feeding rats and hamsters with diets enriched in phospholipids or their precursors, especially ethanolamine, resulted in reduced cholesterol saturation of bile. Although whole phospholipids are normal dietary constituents, the effects and safety of phospholipid components have not been tested in humans. In the present study, we have evaluated the effects of a dietary phospholipid mixture, enriched with phosphatidylethanolamine, on human bile and red blood cell membrane lipid composition. Five ambulatory volunteers having a chronic indwelling T-tube, with an intact enterohepatic circulation, were investigated. Thirty-six grams of phospholipids (54% phosphatidylethanolamine, 54% linoleyl acyl chains) were added to their daily diet for fourteen days. Biliary nucleation time, cholesterol carriers, as well as plasma, red blood cell membrane, and bile lipid compositions, were monitored. Following phospholipid supplementation, the proportion of linoleyl chains (18:2) in biliary phospholipids increased significantly from 31.1±1.2 to 37.7±5.3%, while that of oleyl chains (18:1) decreased from 11.4±1.6 to 9.6±1.1%. These changes were accompanied by an increase of linoleate and its metabolite, arachidonate, in red cell membranes. Phospholipid feeding did not cause any side effects, and no significant changes in biliary nucleation time, cholesterol, phospholipid, or bile salt concentrations, or in the distribution of cholesterol within micelles or vesicles. We conclude that phospholipid feeding is safe, and can be effective as a vehicle for lecithin fatty acyl chain modulation of bile and lipid membranes. These findings may provide a basis for a controlled modulation of biliary phospholipids to increase cholesterol solubility in bile.

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Abbreviations

CSI:

Cholesterol saturation index

FC:

free cholesterol

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

RBC:

red blood cell

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Authors and Affiliations

  1. Sackler Faculty of Medicine, Tel-Aviv University, Ramat Aviv, Petah-Tikva, Israel

    Ronit Pakula, Fred M. Konikoff, Moshe Rubin, Yehuda Ringel, Yochanan Peled & Tuvia Gilat

  2. Department of Surgery “A”, Beilinson Medical Center, Petah-Tikva, Israel

    Moshe Rubin

  3. Felsenstein Medical Research Center, Petah-Tikva, Israel

    Moshe Rubin

  4. Department of Biochemistry, Tel-Aviv University, Ramat Aviv, Israel

    Aliza Tietz

  5. Department of Gastroenterology, Tel Aviv Medical Center, 6 Weizman St., 64239, Tel Aviv, Israel

    Ronit Pakula, Fred M. Konikoff, Yehuda Ringel, Yochanan Peled & Tuvia Gilat

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  1. Ronit Pakula
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Pakula, R., Konikoff, F.M., Rubin, M. et al. The effects of dietary phospholipids enriched with phosphatidylethanolamine on bile and red cell membrane lipids in humans. Lipids 31, 295–303 (1996). https://doi.org/10.1007/BF02529876

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  • DOI: https://doi.org/10.1007/BF02529876

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