Abstract
The release of neurotransmitters principally glutamate during cerebral ischemia has been extensively studied. It is well recognized that ischemia induced release of glutamate plays a key role in “excitotoxic” neuronal death. The role of monoaminergic neurotransmitters is however unclear. The purpose of this study was to evaluate the extracellular norepinephrine, 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) under varied degrees of ischemia in the acute focal ischemic model of the human brain by in-vivo microdialysis. The ischemic response of these amines was correlated with the glutamate levels. Our study concludes that these amines and metabolites can be detected in the human “stroke” model. No marked fluctuations were noted in the levels of norepinephrine and DOPAC. However, significant changes to partial and total ischemia were noted in the extracellular levels of 5-HIAA and 5-HT. These compounds showed a dramatic increase with the onset of ischemia with higher detectable levels in the partial ischemic state in comparison to the total ischemic dialysate levels. The exact role played by the differential increase in the levels of 5-HT to the other catecholamines in the pathogenesis of ischemic neuronal damage remains unclear and warrants further study.
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Bielenberg, G. W., and Burkhardt, M. 1990. 5-Hydroxytryptamine 1A agonists: A new therapeutic principle for stroke treatment. Stroke 21:(suppl IV) 161–163.
Bode-Greuel, K. M., Klisch, J., Horvath, E., Glaser, T., and Traber, J. 1990. Effects of 5-Hydroxytryptamine 1A-Receptor Agonists on Hippocampal Damage After Transient Forebrain Ischemia in the Mongolian Gerbil. Stroke 21:(suppl 4) 164–166.
Donzanti, B. A., and Yamamoto, B. K. 1988. An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates. Life Sci. 43:913–922.
Fujikura, H., Kato, H., Araki, T., Ban, H., Hasegawa, Y., and Kogure, K. 1994. Effects of naftidrofuryl oxalate, a 5-HT2 antagonist, on neuronal damage and local cerebral blood flow following transient cerebral ischemia in gerbils, Brain Res. 636:103–106.
Globus, M. Y.-T., Wester, P., Busto, R., and Dietrich, W. D. 1992. Ischemia-induced extracellular release of serotonin plays a role in CA1 neuronal cell death in rats. Stroke 23:1595–1601.
Hillered, L., Persson, L., Ponten, U., and Ungerstedt, U. 1990. Neurometabolic monitoring of the ischemic human brain using microdialysis. Acta Neurochir. 102:91–97.
Kanthan, R., Shuaib, A., Griebel, R., and Miyashita, H. 1995. Intracerebral human microdialysis: In vivo study of an acute focal ischemic model of the human brain. Stroke 26:870–873.
Kondoh, T., Korosue, K., Lee, S. H., Heros, R. C., and Low, W. C. 1994. Evaluation of monoaminergic neurotransmitters in the rat striatum during varied global cerebral ischemia. Neurosurg. 35: 278–285.
Krieglstein, J., Sauer, D., and Nuglisch, J., Rossberg, C., Beck, T., Bielenberg, W. G. 1989. Naftidrofuryl protects neurons against ischemic damage. Eur. Neurol. 29:224–228.
Nakata, N., Kato, H., and Kogure, K. 1992. Protective effects of serotonin reuptake inhibitors, citalopram and clomipramine, against hippocampal CA1 neuronal damage following transient ischemia in the gerbil. Brain Res. 590:48–52.
Persson, L., and Hillered, L. 1992. Chemical monitoring of neurosurgical intensive care patients using intracerebral microdialysis. J. Neurosurg. 76:72–80.
Ravindran, J., Shuaib, A., Ijaz, S., Galazka, P., Ishaqzay, R., and Miyashita, H. 1994. High extracellular GABA levels in hippocampus—as a mechanism of neuronal protection in cerebral ischemia in adrenalectomized gerbils. Neurosci. Lett. 176:209–211.
Shuaib, A., Ijaz, S., Hemmings, S., Galazka, P., Ishaqzay, R., Ravindran, J., and Miyashita, H. 1994. A decreased glutamate release during hypothyroidism may contribute to protection in cerebral ischemia. Exp. Neurol. 128:260–265.
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Kanthan, R., Shuaib, A., Griebel, R. et al. Evaluation of monoaminergic neurotransmitters in the acute focal ischemic human brain model by intracerebral in vivo microdialysis. Neurochem Res 21, 563–566 (1996). https://doi.org/10.1007/BF02527754
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DOI: https://doi.org/10.1007/BF02527754