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Highly hydrogenated dietary soybean oil modifies the responses to polychlorinated biphenyls in rats

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Lipids

Abstract

The effects of dietary highly hydrogenated soybean oil (HSO) upon the changes caused by dietary polychlorinated biphenyls (PCBs) were examined in rats. Six groups of rats were fed the following diets for 30 d: a 20% soybean oil-containing diet (control diet), a diet in which a half of soybean oil was substituted with HSO (HSO-A diet), a diet in which cellulose powder was replaced with HSO (HSO-B diet) and these diets supplemented with 100 ppm PCBs (control+PCBs, HSO-A+PCBs and HSO-B+PCBs diets). Hepatic concentration of PCBs and relative liver weight were markedly decreased in rats fed with the HSO-A+PCBs diet compared with those fed with the other diets containing PCBs. Liver lipids and liver cholesterol were considerably decreased with a reciprocal increase in fecal sterol excretion by rats fed the HSO-A+PCBs and the HSO-B+PCBs diets compared with those fed with the control+PCBs diet. The fatty acid composition in hepatic phospholipids showed an independent increase of the saturated fatty acid content induced by dietary HSO and PCBs. Dietary PCBs also caused decreases in the amounts of monounsaturated and n-3 polyunsaturated fatty acids. These results suggest that dietary HSO prevents accumulation of PCBs in the liver and promotes the excretion of lipids stimulated by PCBs, accompanied by a change in fatty acid metabolism.

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Abbreviations

HDL:

high density lipoprotein

HSO:

highly hydrogenated soybean oil

MUFA:

monounsaturated fatty acids

PCBs:

polychlorinated biphenyls

PUFA:

polyunsaturated fatty acids

SFA:

saturated fatty acids

References

  1. De Voogt, P., and Brinkman, U.A.T. (1989)Production Properties and Usage of Polychlorinated Biphenyls. Halogenated Biphenyls, Terphenyls, Naphthalenes, Dibenzodioxines and Related Products (Kimbrough, R.D., and Jensen, A.A., eds.) p. 3, Elsevier-North Holland, Amsterdam.

    Google Scholar 

  2. Safe, S.H. (1994) Polychlorinated Biphenyl (PCBs) Environmental Impact, Biochemical and Toxic Responses, and Implications for Risk Assessment,Critical Reviews in Toxicology 24, 87–149.

    PubMed  CAS  Google Scholar 

  3. Smialowicz, R.J., Andrews, J.E., Riddle, M.M., Rogers, R.R., Luebke, R.W., and Copeland, C.B. (1989) Evaluation of the Immunotoxicity of Low Level PCB Exposure in the Rat,Toxicology 56, 197–211.

    Article  PubMed  CAS  Google Scholar 

  4. Barsotti, D.A., Marlar, R.J., and Allen, J.R. (1976) Reproductive Dysfunction in Rheusus Monkeys Exposed to Low Levels of Polychlorinated Biphenyls (Aroclor 1248),Food Cosmet. Toxicol. 14, 99–103.

    Article  PubMed  CAS  Google Scholar 

  5. Horio, F., Ozaki, K., Oda, H., Makino, S., Hayashi, Y., and Yoshida, A. (1987) Effect of Dietary Ascorbic Acid, Cholesterol and PCB on Cholesterol Concentrations in Serum and Liver in a Rat Mutant Unable to Synthesize Ascorbic Acid,J. Nutr. 117, 1036–1044.

    PubMed  CAS  Google Scholar 

  6. Horio, F., Ozaki, K., Oda, H., Makino, S., Hayashi, Y., and Yoshida, A. (1989) Effect of Dietary Ascorbic Acid, Cholesterol and PCB on Cholesterol and Bile Acid Metabolism in a Rat Mutant Unable to Synthesize Ascorbic Acid,J. Nutr. 119, 409–415.

    PubMed  CAS  Google Scholar 

  7. Horio, F., and Yoshida, A. (1982) Effects of Some Xenobiotics on Ascorbic Acid Metabolism in Rats,J. Nutr. 112, 416–425.

    PubMed  CAS  Google Scholar 

  8. Oda, H., Matsuoka, S., and Yoshida, A. (1986) Effects of Dietary Homocysteine and Potassium Sulfate on Serum Cholesterol and Urinary Ascorbic Acid in Rats Fed PCB,J. Nutr. 116, 1660–1666.

    PubMed  CAS  Google Scholar 

  9. Kato, N., Kato, M., Kimura, T., and Yoshida, A. (1978) Effect of Dietary Addition of PCB, DDT, or BHC and Dietary Protein on Vitamin A and Cholesterol Metabolism,Nutr. Rep. Internat. 18, 437–445.

    CAS  Google Scholar 

  10. Kawai-Kobayashi, K., and Yoshida, A. (1986) Effect of Dietary Ascorbic Acid and Vitamin E on Metabolic Changes in Rats and Guinea Pigs Exposed to PCB,J. Nutr. 116, 98–106.

    PubMed  CAS  Google Scholar 

  11. Kato, N., and Yoshida, A. (1981) Effect of Various Dietary Xenobiotics on Serum Total Cholesterol and High Density Lipoprotein Cholesterol in Rats,Nurt. Rep. Internat. 23, 825–831.

    CAS  Google Scholar 

  12. Kamei, M., Fujita, T., Kanbe, T., Odachi, J., Sasaki, K., Otani, S., Matsui-Yuasa, I., and Morisawa, S. (1987) Effects of Ubiquinone-10 on Several Metabolic Responses in Rats Fed PCB Containing Diets,Ann. Rep. Osaka City Inst. Pub. Health and Environ. Sci. 49, 93–97.

    Google Scholar 

  13. Kamei, M., Kanbe, T., Fujita, T., Sasaki, K., Otani, S., Matsui-Yuasa, I., and Morisawa, S. (1987) Effects of Dietary PCB and/or Cholesterol on Metabolic Activity in Rats,Ann. Rep. Osaka City Inst. Pub. Health and Environ. Sci., 50, 15–20.

    Google Scholar 

  14. Kamei, M., Kanbe, T., Yamada, A., and Morita, S. (1992) Effects of Maccha (powdered green tea) Intake on Altered Cholesterol Metabolism by Dietary PCB in Rats,Ann. Rep. Osaka City Inst. Pub. Health and Environ. Sci. 54, 57–58.

    Google Scholar 

  15. Kamei, M., Ogaki, S., Kanbe, T., Niiya, I., Mizutani, H., Matsui-Yuasa, I., Otani, S., and Morita, S. (1995) Effects of Highly Hydrogenated Soybean Oil and Cholesterol on Plasma, Liver Cholesterol and Fecal Steroids in Rats,Lipids 30, 533–539.

    PubMed  CAS  Google Scholar 

  16. Ukawa, M., Nakamura, A., and Kashimoto, T. (1973) Studies on a Calculation Method for Polychlorinated Biphenyl (PCB) Isomers,J. Food Hyg. Soc. Japan 14, 415–424.

    Google Scholar 

  17. Folch, J., Lees, M., and Sloane Stanley, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissue,J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  18. Grundy, S.M., Ahrens, E.H., Jr., and Miettinen, T.A. (1965) Quantitative Isolation and Gas-Liquid Chromatographic Analysis of Total Fecal Bile Acids,J. Lipid Res. 6, 397–410.

    PubMed  CAS  Google Scholar 

  19. Yamanaka, Y., Tsuji, K., and Ichikawa, T. (1986) Stimulation of Chemodeoxycholic Acid Excretion in Hypercholesterolemic Mice by Dietary Taurine,J. Nutr. Sci. Vitaminol. 32, 287–296.

    PubMed  CAS  Google Scholar 

  20. Forman, D.T., Philips, C., Walter, E., and Taylor, C.B. (1968) Fluorometric Measurement of Fecal Bile Acids,Clin. Chem. 14, 348–359.

    PubMed  CAS  Google Scholar 

  21. Saito, K., and Gamo, M. (1986) Distribution of Unusual Fatty Acids in Preen Gland Lipids,J. Biochem. (Tokyo) 64, 557–559.

    Google Scholar 

  22. Kawai, N., and Nakayama, Y. (1985) Lipids of Fishes I., Lipid Content, Fatty Acid Composition and Cholesterol Content of Teleost Fishes,Ann. Rep. Osaka City Inst. Pub. Health and Environ. Sci. 48, 48–164.

    Google Scholar 

  23. Okamura, M. (1981) A Specific Method for Determination of Total Ascorbic Acids in Urine by the α, α′-Diphyridyl Method,Clin. Chim. Acta 115, 393–403.

    Article  PubMed  CAS  Google Scholar 

  24. Omura, T., and Sato, R. (1964) The Carbon Monoxide Binding Pigment of Liver Microsomes,J. Biol. Chem. 239, 2370–2378.

    PubMed  CAS  Google Scholar 

  25. Lillie, R.D. (1944) Various Oil Soluble Dyes as Fat Stains in the Supersaturated Isopropanol Technic,Stain Tech. 19, 55–58.

    Google Scholar 

  26. Mattson, F.H. (1959) The Absorbability of Stearic Acid When Fed as a Simple or Mixed Triglyceride,J. Nutr. 69, 338–342.

    CAS  Google Scholar 

  27. Mottil, G.E., Brinkley, A.W., and Mersinger, C.L. (1980) Biochemical Characterization of Cholesterol-ReducingEubacterium, Appl. Environ. Microbiol. 40, 1017–1022.

    Google Scholar 

  28. Itokawa, Y., Yagi, N., Kaito, H., Kamohara, K., and Fujiwara, K. (1976) Influence of Diet on the Induction of Hepatic Ceroid Pigment in Rats by Polychlorinated Biphenyls,Toxicol. Appl. Pharmacol. 36, 131–141.

    Article  PubMed  CAS  Google Scholar 

  29. Leikin, A.I., and Brenner, R.R. (1987) Cholesterol-Induced Microsomal Changes Modulate Desaturase Activities,Biochim. Biophys. Acta 922, 294–303.

    PubMed  CAS  Google Scholar 

  30. Borlakoglu, T., Edwards-Webb, J.D., and Dils, R.R. (1990) Polychlorinated Biphenyls Increase Fatty Acid Desaturation in the Proliferating Endoplasmic Reticulum of Pigeon and Rat Liver,Eur. J. Biochem. 188, 327–332.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Nutritional Physiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, 543, Osaka, Japan

    Masaharu Kamei, Sumiko Ohgaki, Mitsuru Shimizu & Shigeru Morita

  2. Department of Domestic Sciences, Faculty of Education, Miyazaki University, 889-21, Miyazaki, Japan

    Tamotsu Kanbe

  3. Japan Institute of Oil and Fats, Other Foods Inspection Foundation, 103, Tokyo, Japan

    Isao Niiya

  4. Department of Food and Nutrition, Faculty of Human Life Science, Osaka City University, 558, Osaka, Japan

    Isao Matsui-Yuasa

  5. Department of Biochemistry, Osaka City University, Medical School, 545, Osaka, Japan

    Shuzo Otani

Authors
  1. Masaharu Kamei
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  2. Sumiko Ohgaki
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  3. Tamotsu Kanbe
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  4. Mitsuru Shimizu
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  5. Shigeru Morita
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  6. Isao Niiya
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  7. Isao Matsui-Yuasa
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  8. Shuzo Otani
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Kamei, M., Ohgaki, S., Kanbe, T. et al. Highly hydrogenated dietary soybean oil modifies the responses to polychlorinated biphenyls in rats. Lipids 31, 1151–1156 (1996). https://doi.org/10.1007/BF02524289

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  • DOI: https://doi.org/10.1007/BF02524289

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