Abstract
Determination of the content of vernolic acid (12,13-epoxy-9c-octadecenoic) in the oil ofEuphorbia lagascae has been performed by gas chromatography of the fatty acid methyl ester derivatives of the triacylglycerols in the oil and by supercritical fluid chromatography (SFC) of the raw oil and the fatty acid derivatives of the oil. The content of vernolic acid was found to be 55 wt%. The three methods were compared, and SFC analysis of the fatty acid derivatives was found to be the most accurate method.
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Borch-Jensen, C., Mollerup, J. Determination of vernolic acid content in the oil ofEuphorbia lagascae by gas and supercritical fluid chromatography. J Am Oil Chem Soc 73, 1161–1164 (1996). https://doi.org/10.1007/BF02523378
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DOI: https://doi.org/10.1007/BF02523378