Abstract
Immobilization of r-CHO cells at high density using macroporous polyethylene carriers in a modular fluidized bed reactor is demonstrated. Specific growth rates of the cells are measured by incorporation of BrdU. At a cell density of about 108 cells/ml a stable growth rate of 0.004 h−1 was established. Total release of proteins into the culture supernatant during protein-free perfusion was analyzed by 2-DE in various phases of the long-term culture showing very similar patterns indicating a constant pattern of gene expression.
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Abbreviations
- BrdU:
-
Bromodeoxyuridine
- 2-DE:
-
Two-Dimensional Electrophoresis
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Reiter, M., Borth, N., Blüml, G. et al. Flow cytometry and two-dimensional electrophoresis (2-DE) for system evaluation of long term continuous perfused animal cell cultures in macroporous beads. Cytotechnology 9, 247–253 (1992). https://doi.org/10.1007/BF02521752
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DOI: https://doi.org/10.1007/BF02521752