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The protein of glial intermediate filaments in different areas of the rat brain at experimental neurosis

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Abstract

The content and polypeptide composition of glial fibrillary acidic protein (GFAP) in the rat cerebral cortex, cerebellum, hippocampus, and mesencephalon were studied under conditions of experimental neurosis. Significant changes of the total GFAP content were observed in the hippocampus, mesencephalon, and cerebellum. Both the content and polypeptide composition of soluble GFAP form were markedly modified. These changes of glial filament protein apparently reflect the peculiarities of the reorganization of the astrocyte intermediate filaments at the animal’s long-term neurotization.

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References

  1. V. A. Berezin, G. M. Shevchenko, G. G. Bunatyan et al., “Specific proteins of intermediate filaments in normal neural tissue and in brain tumors,”Neurochemistry,6, No. 2, 77–82 (1987).

    CAS  Google Scholar 

  2. N. J. Messenger, and A. E. Warner, “The appearance of neuronal and glial markers during early development of the nervous system,”Development,10, No. 1, 43–54 (1989).

    Google Scholar 

  3. N. Funayama, F. Fagotto, P. McCrea, and B. M. Gumbiner, “Embryonic axis induction by the armadillo repeat domain of beta-catenin: evidence for intracellular signaling,”J. Cell Biol.,128, No. 5, 953–968 (1995).

    Article  Google Scholar 

  4. O. L. Berezovskaya, D. A. Rusakov, G. G. Skibo, et al., “Interaction of neurofilaments and mitochondria in cultured cells of rat hippocampus,”Neirofiziologiya/Neurophysiology,27, No. 1, 1–6 (1995).

    Google Scholar 

  5. P. H. Mangeat, “Interaction of biological membrane with the cytoskeletal framework of living cells,”J. Biol. Cell,64, 261–281 (1988).

    Article  CAS  Google Scholar 

  6. V. S. Nedzvetski, V. A. Berezin, T. I. Obernyak, and E. N. Zhmaryova, “Characteristics of specific proteins of the intermediate filaments in the human brain tumors,”Biokhimiya,51, No. 11, 1843–1850 (1986).

    Google Scholar 

  7. O. H. Lowry, H. I. Rosenbrough, Z. A. Farr, and R. Z. Randell, “Protein measurement with the folin phenol reagent,”J. Biol. Chem.,193, 265–278 (1955).

    Google Scholar 

  8. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,”Nature,227, No. 1, 249–256 (1970).

    Google Scholar 

  9. V. S. Nedzvetski, B. A. Berezin, A. I. Dvoretski, and S. G. Busygina, “CNS-syndrome: characteristics of glial intermediate filaments in rat brain at total X-irradiation,”Radiobiologiya,30, Issue 2, 243–246 (1990).

    Google Scholar 

  10. F. P. Vedyaev,Zh. Vyssh. Nerv. Deyat., No. 2, 325–327 (1977).

    Google Scholar 

  11. N. L. Banik, R. D. Happel, M. B. Sostek, et al., “Ca-mediated degradation of central nervous system proteins: topographic and species variation,”Metab. Brain Dis.,2, No. 2, 117–126 (1987).

    Article  PubMed  CAS  Google Scholar 

  12. L. Eng, J. Vanderhnegher, A. Bignami, and B. Gerst, “Acidic protein from fibrous astrocytes,”Brain Res.,971, 352–356 (1982).

    Google Scholar 

  13. A. Paetau, and I. Virtanen, “Cytoskeletal properties and endogenous degradation of glial fibrillary acidic protein and vimentin in cultured human glioma cells,”Acta Neuropathol. Berl.,69, 73–80 (1986).

    Article  PubMed  CAS  Google Scholar 

  14. V. S. Nedzvetskii, G. A. Ushakova, S. G. Busygina et al., “Effect of small doses of ionizing radiation on intermediate filaments and Ca-activated system of the rat brain proteolysis,”Radiobiologiya,31, issue 3, 333–339 (1991).

    CAS  Google Scholar 

  15. J. F. Conway and D. A. D. Parry, “Intermediate filaments structure: analysis of sequence homologies,”J. Biol. Macromol.,10, 79–98 (1988).

    Article  CAS  Google Scholar 

  16. E. Wu, and C. S. Raine, “Multiple sclerosis. Interaction between oligodendrocytes and hypertrophic astrocytes and their occurrence in other nondemyelinating conditions,”Lab. Invest.,67, No. 1, 88–99 (1992).

    PubMed  CAS  Google Scholar 

  17. M. M. Kozlovskaya, V. V. Rozhanets, A. Ya. Mekhedova, and A. V. Val’dman, in:Molecular Base of the Action of Psychotropic Drugs, NII Farmakologii AMN SSSR, Moscow (1986), pp. 22–32.

    Google Scholar 

  18. G. L. Hatton, S. M. Luckman, and R. J. Bicknell, “Adrenaline activation of beta2-adrenoreceptors stimulates morphological changes in astrocytes cultured from adult rat neurohypophysis,”Brain Res. Bull.,26, No. 5, 765–769 (1991).

    Article  PubMed  CAS  Google Scholar 

  19. C. Giaume, P. Marin, J. Cordier, and J. Glovinski, “Adrenergic regulation of intercellular communications between cultured striatal astrocytes from the mouse,”Proc. Natl. Acad. Sci. USA,88, No. 13, 5577–5581 (1991).

    Article  PubMed  CAS  Google Scholar 

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Nedzvetskii, V.S., Nerush, P.A. The protein of glial intermediate filaments in different areas of the rat brain at experimental neurosis. Neurophysiology 31, 94–97 (1999). https://doi.org/10.1007/BF02515043

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