Abstract
The content and polypeptide composition of glial fibrillary acidic protein (GFAP) in the rat cerebral cortex, cerebellum, hippocampus, and mesencephalon were studied under conditions of experimental neurosis. Significant changes of the total GFAP content were observed in the hippocampus, mesencephalon, and cerebellum. Both the content and polypeptide composition of soluble GFAP form were markedly modified. These changes of glial filament protein apparently reflect the peculiarities of the reorganization of the astrocyte intermediate filaments at the animal’s long-term neurotization.
Similar content being viewed by others
References
V. A. Berezin, G. M. Shevchenko, G. G. Bunatyan et al., “Specific proteins of intermediate filaments in normal neural tissue and in brain tumors,”Neurochemistry,6, No. 2, 77–82 (1987).
N. J. Messenger, and A. E. Warner, “The appearance of neuronal and glial markers during early development of the nervous system,”Development,10, No. 1, 43–54 (1989).
N. Funayama, F. Fagotto, P. McCrea, and B. M. Gumbiner, “Embryonic axis induction by the armadillo repeat domain of beta-catenin: evidence for intracellular signaling,”J. Cell Biol.,128, No. 5, 953–968 (1995).
O. L. Berezovskaya, D. A. Rusakov, G. G. Skibo, et al., “Interaction of neurofilaments and mitochondria in cultured cells of rat hippocampus,”Neirofiziologiya/Neurophysiology,27, No. 1, 1–6 (1995).
P. H. Mangeat, “Interaction of biological membrane with the cytoskeletal framework of living cells,”J. Biol. Cell,64, 261–281 (1988).
V. S. Nedzvetski, V. A. Berezin, T. I. Obernyak, and E. N. Zhmaryova, “Characteristics of specific proteins of the intermediate filaments in the human brain tumors,”Biokhimiya,51, No. 11, 1843–1850 (1986).
O. H. Lowry, H. I. Rosenbrough, Z. A. Farr, and R. Z. Randell, “Protein measurement with the folin phenol reagent,”J. Biol. Chem.,193, 265–278 (1955).
U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,”Nature,227, No. 1, 249–256 (1970).
V. S. Nedzvetski, B. A. Berezin, A. I. Dvoretski, and S. G. Busygina, “CNS-syndrome: characteristics of glial intermediate filaments in rat brain at total X-irradiation,”Radiobiologiya,30, Issue 2, 243–246 (1990).
F. P. Vedyaev,Zh. Vyssh. Nerv. Deyat., No. 2, 325–327 (1977).
N. L. Banik, R. D. Happel, M. B. Sostek, et al., “Ca-mediated degradation of central nervous system proteins: topographic and species variation,”Metab. Brain Dis.,2, No. 2, 117–126 (1987).
L. Eng, J. Vanderhnegher, A. Bignami, and B. Gerst, “Acidic protein from fibrous astrocytes,”Brain Res.,971, 352–356 (1982).
A. Paetau, and I. Virtanen, “Cytoskeletal properties and endogenous degradation of glial fibrillary acidic protein and vimentin in cultured human glioma cells,”Acta Neuropathol. Berl.,69, 73–80 (1986).
V. S. Nedzvetskii, G. A. Ushakova, S. G. Busygina et al., “Effect of small doses of ionizing radiation on intermediate filaments and Ca-activated system of the rat brain proteolysis,”Radiobiologiya,31, issue 3, 333–339 (1991).
J. F. Conway and D. A. D. Parry, “Intermediate filaments structure: analysis of sequence homologies,”J. Biol. Macromol.,10, 79–98 (1988).
E. Wu, and C. S. Raine, “Multiple sclerosis. Interaction between oligodendrocytes and hypertrophic astrocytes and their occurrence in other nondemyelinating conditions,”Lab. Invest.,67, No. 1, 88–99 (1992).
M. M. Kozlovskaya, V. V. Rozhanets, A. Ya. Mekhedova, and A. V. Val’dman, in:Molecular Base of the Action of Psychotropic Drugs, NII Farmakologii AMN SSSR, Moscow (1986), pp. 22–32.
G. L. Hatton, S. M. Luckman, and R. J. Bicknell, “Adrenaline activation of beta2-adrenoreceptors stimulates morphological changes in astrocytes cultured from adult rat neurohypophysis,”Brain Res. Bull.,26, No. 5, 765–769 (1991).
C. Giaume, P. Marin, J. Cordier, and J. Glovinski, “Adrenergic regulation of intercellular communications between cultured striatal astrocytes from the mouse,”Proc. Natl. Acad. Sci. USA,88, No. 13, 5577–5581 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nedzvetskii, V.S., Nerush, P.A. The protein of glial intermediate filaments in different areas of the rat brain at experimental neurosis. Neurophysiology 31, 94–97 (1999). https://doi.org/10.1007/BF02515043
Issue Date:
DOI: https://doi.org/10.1007/BF02515043