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Immunocytochemical and chemical analyses of Golgi vesicles isolated from the germinated pollen ofCamellia japonica

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Abstract

As a first step towards studying the biochemical relationship between Golgi vesicles (GVs) and tube wall components, isolation of GVs from the growing pollen tubes ofCamellia japonica was attempted using a centrifugation method with mannitol. The isolated GV was identified ultrastructurally and immunocytochemically. The main components of the GV were proteins and carbohydrates. The main monosaccharides of GV polysaccharides were galactose, arabinose and uronic acid, and pectins and arabinogalactan proteins also were detected immunochemically. An antiserum against the isolated GVs reacted with the outer layer of the pollen tube wall and the intine layers of the grain wall as well as thein situ GVs in the pollen tube and the grain cytoplasm. We have thus successfully isolated GVs and shown that they contain pectic substances and arabinogalactan proteins which contribute to formation of the pollen tube primary wall.

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Authors and Affiliations

  1. Graduate School of Integrated Science, Yokohama City University, 236, Yokohama, Japan

    Yoshikazu Hasegawa & Norio Nakamura

  2. Biological Laboratory, Kanagawa Dental College, 238, Yokosuka, Japan

    Sumio Nakamura

  3. Department of Biology, Yokohama City, University, Kanazawa-ku, 236, Yokohama, Japan

    Sayuri Kakizoe, Masayuki Sato & Norio Nakamura

Authors
  1. Yoshikazu Hasegawa
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  2. Sumio Nakamura
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  3. Sayuri Kakizoe
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  4. Masayuki Sato
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  5. Norio Nakamura
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Correspondence to Norio Nakamura.

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Hasegawa, Y., Nakamura, S., Kakizoe, S. et al. Immunocytochemical and chemical analyses of Golgi vesicles isolated from the germinated pollen ofCamellia japonica . J. Plant Res. 111, 421–429 (1998). https://doi.org/10.1007/BF02507807

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  • DOI: https://doi.org/10.1007/BF02507807

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