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LC analysis of biogenic polyamines in table olives using on-line dansylation and peroxyoxalate chemiluminescence detection

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Summary

Biogenic polyamines were determined at femtomole concentrations in table olives using an on-line, liquid-chromatographic system involving dansylation and peroxyoxalate chemiluminescence-based detection. Samples simply obtained by treating table olives with trichloroacetic acid are derivatized on-line with dansyl chloride and 0.6 M borate buffer (pH 11), are separated on a C18 analytical column and determined using an integrated derivatization chemiluminescence detection unit based on the bis(2,4,6,-trichlorophenyl)oxalate-hydrogen peroxide system. Analytes can thus be determined in the concentration range 0.2–150 pmol (mass of analyte injected into chromatograph), with relative standard deviations 1.9–3.7% and limits of detection in the range 75–150 fmol. The proposed method surpasses other LC alternatives based on different detection techniques in terms of limits of detection.

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Authors and Affiliations

  1. Analytical Chemistry Division, Department of Analytical Chemistry and Ecology, University of Córdoba, 14004, Córdoba, Spain

    M. Cobo & M. Silva

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  1. M. Cobo
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  2. M. Silva
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Cobo, M., Silva, M. LC analysis of biogenic polyamines in table olives using on-line dansylation and peroxyoxalate chemiluminescence detection. Chromatographia 51, 706–712 (2000). https://doi.org/10.1007/BF02505409

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  • DOI: https://doi.org/10.1007/BF02505409

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